李栋, 李平亮, 李保华, 董向丽. QuEChERS-高效液相色谱法分析6-苄氨基腺嘌呤和吡唑醚菌酯在番茄植株和土壤中的残留动态[J]. 农药学学报, 2020, 22(1): 97-104. DOI: 10.16801/j.issn.1008-7303.2020.0006
    引用本文: 李栋, 李平亮, 李保华, 董向丽. QuEChERS-高效液相色谱法分析6-苄氨基腺嘌呤和吡唑醚菌酯在番茄植株和土壤中的残留动态[J]. 农药学学报, 2020, 22(1): 97-104. DOI: 10.16801/j.issn.1008-7303.2020.0006
    LI Dong, LI Pingliang, LI Baohua, DONG Xiangli. Residue kinetics analysis of 6-benzylaminopurine and pyraclostrobin in tomato plants and soils by QuEChERS-high performance liquid chromatography[J]. Chinese Journal of Pesticide Science, 2020, 22(1): 97-104. DOI: 10.16801/j.issn.1008-7303.2020.0006
    Citation: LI Dong, LI Pingliang, LI Baohua, DONG Xiangli. Residue kinetics analysis of 6-benzylaminopurine and pyraclostrobin in tomato plants and soils by QuEChERS-high performance liquid chromatography[J]. Chinese Journal of Pesticide Science, 2020, 22(1): 97-104. DOI: 10.16801/j.issn.1008-7303.2020.0006

    QuEChERS-高效液相色谱法分析6-苄氨基腺嘌呤和吡唑醚菌酯在番茄植株和土壤中的残留动态

    Residue kinetics analysis of 6-benzylaminopurine and pyraclostrobin in tomato plants and soils by QuEChERS-high performance liquid chromatography

    • 摘要: 为了探究根部处理剂6-苄氨基腺嘌呤 (6-BA) 和吡唑醚菌酯在植物-土壤间的分布规律,以番茄为试材,建立了测定番茄根、叶及根围土壤中2种化合物的QuEChERS-高效液相色谱法,并采用该方法研究了两者在番茄体内和根围土壤中28 d内的含量变化。结果表明:6-BA与吡唑醚菌酯在番茄根、叶和根围土壤中的回收率在82% ~ 107%之间,定量限在0.03 ~ 0.09 mg/kg之间;经番茄苗蘸根处理后12 h ~ 28 d,在根围土壤中未检测到6-BA,而在番茄叶和根中均有检出 (0.043 ~ 2.0 mg/kg),表明6-BA可被根部快速吸收,并传导至叶片,在番茄各部位中的含量为侧叶>根部>顶叶;6-BA在番茄根部和叶片中消解较快,处理7 ~ 10 d后低于检出限;吡唑醚菌酯可扩散至根围土壤中,大部分被根部吸收并维持在较高含量水平 (1.2 ~ 3.0 mg/kg) 达28 d以上;根部的吡唑醚菌酯可转移至叶片,并以较低含量水平 (0.11 ~ 0.78 mg/kg) 保留4 ~ 6 d。研究结果为番茄根、叶及土壤中6-BA和吡唑醚菌酯的残留分析提供了方法,并为评价该根部处理剂的应用效果提供了必要数据。

       

      Abstract: In order to investigate the distribution behavior of 6-benzylaminopurine (6-BA) and pyraclostrobin (two active ingredients for root-treatment agent) in plants, tomato was used as a model plant. A QuEChERS-high performance liquid chromatography method was developed to determine their dynamic contents in tomato roots, leaves and the rhizosphere soils. Results showed that the recoveries of 6-BA and pyraclostrobin in tomato and the rhizosphere soil were in the range of 82%-107% and the quantitative limit (LOQ) range was 0.03-0.09 mg/kg. After the root treatment, there was no 6-BA detected in the soil, but it was detected in the tomato roots and leaves in the range from 0.043 to 2.0 mg/kg, indicating the bio-availability by plants was very high. 6-BA can be quickly absorbed by the roots and transferred to the side-leaf and the top-leaf. The order of distribution levels is side-leaf > root > top-leaf. 6-BA degraded at a high speed in both tomato roots and the leaves. Thus, it was not detected 7-10 days after the treatment. A proportion of pyraclostrobin was distributed into the soil and most of that proportion was absorbed by roots with a high level (1.2-3.0 mg/kg) for 28 days. A small amount of pyraclostrobin in roots was transferred to leaves and was kept with a level of 0.11-0.78 mg/kg for 4-6 days. This study has developed a method for the residue analysis of 6-BA and pyraclostrobin in the tomato root, leaf and soil, and it has also provided necessary data for evaluating the application of the root-treatment agent.

       

    /

    返回文章
    返回