陈运泽, 张博文, 吴德东, 曾健勇, 杨璟, 张国财. 响应面优化烟草提取物微囊制备工艺[J]. 农药学学报, 2020, 22(3): 534-542. DOI: 10.16801/j.issn.1008-7303.2020.0038
    引用本文: 陈运泽, 张博文, 吴德东, 曾健勇, 杨璟, 张国财. 响应面优化烟草提取物微囊制备工艺[J]. 农药学学报, 2020, 22(3): 534-542. DOI: 10.16801/j.issn.1008-7303.2020.0038
    CHEN Yunze, ZHANG Bowen, WU Dedong, ZENG Jianyong, YANG Jing, ZHANG Guocai. Response surface optimization of microcapsule preparation of tobacco (Nicotiana tabacum L.) extract[J]. Chinese Journal of Pesticide Science, 2020, 22(3): 534-542. DOI: 10.16801/j.issn.1008-7303.2020.0038
    Citation: CHEN Yunze, ZHANG Bowen, WU Dedong, ZENG Jianyong, YANG Jing, ZHANG Guocai. Response surface optimization of microcapsule preparation of tobacco (Nicotiana tabacum L.) extract[J]. Chinese Journal of Pesticide Science, 2020, 22(3): 534-542. DOI: 10.16801/j.issn.1008-7303.2020.0038

    响应面优化烟草提取物微囊制备工艺

    Response surface optimization of microcapsule preparation of tobacco (Nicotiana tabacum L.) extract

    • 摘要: 烟草提取物具有良好的杀虫活性。为提高其稳定性,以烟草提取物为芯材,以明胶、阿拉伯胶为壁材,采用复凝聚法制备了烟草提取物微囊。以包埋率为评价指标,采用响应面法对微囊制备工艺进行优化。对微囊进行了表面形态观察、粒径测定、红外光谱分析、热稳定性分析及室内毒力测定。结果表明:当乳化剂质量分数4%、明胶质量分数2.05%、阿拉伯胶质量分数3%、壁芯质量比1.34 : 1.00以及复凝聚反应时间为36 min时,制备的微囊包埋率最高,理论包埋率为58.93%。经验证,在此条件下实际包埋率为58.42% ± 0.12%,接近理论值。所得微囊表面光滑,成囊性好,粒径分布在4.72~17.05 μm之间,中位径8.63 μm;在877.55和2 922.13 cm−1处保留了烟草提取物的红外特征峰;微囊化能够提高烟草提取物的热稳定性;室内毒力测定结果表明,烟草提取物微囊的LC50值为20.19 mg/mL,与烟草提取物的毒力水平相当,说明微囊化没有降低烟草提取物的毒力。本研究可以为烟草提取物微囊制备提供参考。

       

      Abstract: Tobacco extract has good insecticidal activity. In order to improve the stability of tobacco extract, microcapsules were prepared by complex coacervation with tobacco extract as the core material and gelatin and gum arabic as the wall material. The preparation method of microcapsules was optimized by the response surface method with the embedding rate as the evaluation index. The surface morphology, the particle size measurement, the infrared spectrum analysis, the thermal stability analysis and the indoor virulence determination of microcapsules were carried out. The results showed the highest embedding rate of 58.93% was obtained when the following conditions were used: emulsifier mass fraction 4%, gelatin mass fraction 2.05%, gum arabic mass fraction 3%, wall core mass ratio 1.34 : 1.00 and complex coacervation reaction time of 36 min. The actual embedding rate of microcapsules under these conditions was 58.42% ± 0.12%, which was close to the theoretical value. The microcapsules prepared by this model exhibted smooth surface, good capsule genicity with the particle size distribution of 4.72-17.05 μm and median diameter of 8.63 μm.Infrared characteristic peaks of tobacco extracts were preserved at 877.55 cm−1 and 2 922.13 cm−1. Microencapsulation can improve the thermal stability of tobacco extract. Indoor virulence test showed that the LC50 value of tobacco extract microcapsules was 20.19 mg/mL, which was equivalent to the virulence level of the tobacco extract, indicating that microencapsulation didn’t reduce the insecticidal effect of tobacco extracts. To conclude, the present study can provide a reference for the optimization of tobacco extract microcapsules preparation process.

       

    /

    返回文章
    返回