许春丽, MuhammadBILAL, 徐博, 冉刚超, 赵鹏跃, 曹冲, 李凤敏, 曹立冬, 黄啟良. 荧光介孔二氧化硅负载丙硫菌唑纳米颗粒的制备及性能研究[J]. 农药学学报, 2020, 22(2): 214-224. DOI: 10.16801/j.issn.1008-7303.2020.0046
    引用本文: 许春丽, MuhammadBILAL, 徐博, 冉刚超, 赵鹏跃, 曹冲, 李凤敏, 曹立冬, 黄啟良. 荧光介孔二氧化硅负载丙硫菌唑纳米颗粒的制备及性能研究[J]. 农药学学报, 2020, 22(2): 214-224. DOI: 10.16801/j.issn.1008-7303.2020.0046
    XU Chunli, Muhammad BILAL, XU Bo, RAN Gangchao, ZHAO Pengyue, CAO Chong, LI Fengmin, CAO Lidong, HUANG Qiliang. Preparation and characterization of prothioconazole-loaded fluorescent mesoporous silica nanoparticles[J]. Chinese Journal of Pesticide Science, 2020, 22(2): 214-224. DOI: 10.16801/j.issn.1008-7303.2020.0046
    Citation: XU Chunli, Muhammad BILAL, XU Bo, RAN Gangchao, ZHAO Pengyue, CAO Chong, LI Fengmin, CAO Lidong, HUANG Qiliang. Preparation and characterization of prothioconazole-loaded fluorescent mesoporous silica nanoparticles[J]. Chinese Journal of Pesticide Science, 2020, 22(2): 214-224. DOI: 10.16801/j.issn.1008-7303.2020.0046

    荧光介孔二氧化硅负载丙硫菌唑纳米颗粒的制备及性能研究

    Preparation and characterization of prothioconazole-loaded fluorescent mesoporous silica nanoparticles

    • 摘要: 近年来,丙硫菌唑作为一种新颖的广谱杀菌剂备受关注,但由于其代谢产物对操作人员存在健康风险,导致其在中国的使用和登记受到限制。将丙硫菌唑制备为具有缓慢释放、可提高农药稳定性等特点的缓释制剂可有效改善其带来的健康风险问题。本研究采用纳米荧光介孔二氧化硅制备了丙硫菌唑缓释纳米颗粒,通过扫描电镜(SEM)、透射电镜(TEM)、傅里叶变换红外光谱(FTIR)、X-射线光电子能谱(XPS)、Brunauer-Emmett-Teller (BET)比表面积分析、热重分析(TGA)、荧光光谱以及高效液相色谱仪等手段对其结构与特性进行表征。采用透析袋法探讨了丙硫菌唑在释放介质中的释放行为;利用菌丝生长速率法测定了该载药颗粒对小麦赤霉病原菌的杀菌活性;通过激光共聚焦研究了荧光二氧化硅在小麦赤霉病菌菌丝体以及小麦中的传输情况。结果表明:所制备的载药纳米颗粒外观形貌较为规整,平均粒径为139 nm,其最大载药量可达27.14%;该纳米载药颗粒具有缓释性能,释放曲线符合Ritger-Peppas方程;载药颗粒与丙硫菌唑原药相比具有相同的毒力效果。此外,激光共聚焦结果表明,该荧光二氧化硅纳米颗粒可以在菌丝体以及小麦植株根部吸收和传导。

       

      Abstract: Recently, as a novel broad-spectrum fungicide, prothioconazole has attracted much attention. However, the use and registration of prothioconazole in China were restricted due to its metabolites which has great health risks to operators. Therefore, an effectively method to decrease its toxicity is the preparation of the slow release formulation. In this work, the slow release system of prothioconazole-loaded fluorescent mesoporous nanoparticles was prepared. The surface morphology and structure were characterized and analyzed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Brunauer-Emmett-Teller (BET) surface analysis, thermogravimetric analysis (TGA) and high performance liquid chromatography (HPLC). The release behavior of nanoparticles was evaluated by the dialysis bag method. The growth rate method was employed to study the virulence against Fusahum graminearum Sehw. The optical properties was investigated by fluorescence spectrometer and the transmission of the nanoparticles in fungi and wheat plant were characterized by confocal laser scanning microscopy (CLSM). The results showed that the prepared pesticide-loaded nanoparticles (Pro@FL-MSN) exhibited regular appearance with the maximum drug loading of 27.14% and the average particle size was 139 nm. The results of the release study indicated that the release rate was consistent with the Ritger-Peppas equation. The fungicidal activity of Pro@FL-MSN was similar to that of prothioconazole technical. Moreover, CLSM results showed that the fluorescent silica nanoparticles could be absorbed and transfer into the mycelium and the roots of wheat plant.

       

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