Abstract: Quinotrione is a new HPPD herbicide. In order to determine the feasibility of quinotrione in wheat field, its activity against 22 weeds and its safety to three wheat varieties in three application periods (pre-emergence, early of post-emergence and post-emergence) were measured in the greenhouse. At the same time, the field experiments of quinotrione in wheat field were carried out. The results of greenhouse experiments showed that quinotrione was safe to wheat in three periods, even at the highest dose of 720 g a.i./hm². Quinotrione was efficient for the control of Veronica persica and Silene conoidea. In terms of Veronica persica, the GR₅₀ of post-emergence treatment was 1.4-5.4 g a.i./hm², and the GR₉₀ was 6.8-14.2 g a.i./hm². In terms of Silene conoidea, the GR₅₀ of pre-emergence treatment was 13.3-13.8 g a.i./hm², and the GR₉₀ was 47.7-64.0 g a.i./hm². In addition, it had positive effect on Galium aparine, Lithospermum arvense and Descurainia sophia with GR₅₀ 7.3-98.9 g a.i./hm² by post-emergence spraying. Two years field experiments showed that quinotrione was safe to wheat and effective to the weeds in the field, and the control efficiencies of quinotrione against V. persica and Silene conoidea were both nearly 100%. It also had certain effect on D. sophia and G. aparine with fresh weight control efficiencies of 65.3%-80.1%. The greenhouse and field tests showed that quetrione had unique advantages in wheat field. The object of our future research is to find out the suitable herbicides for mix using and expanding the control spectrum.