冯明星, 胡兆农, 吴文君. 杠柳新苷类化合物对东方黏虫的杀虫活性及对中肠细胞刷状缘膜囊泡3种特征酶活性的影响[J]. 农药学学报, 2021, 23(2): 308-315. DOI: 10.16801/j.issn.1008-7303.2021.0003
    引用本文: 冯明星, 胡兆农, 吴文君. 杠柳新苷类化合物对东方黏虫的杀虫活性及对中肠细胞刷状缘膜囊泡3种特征酶活性的影响[J]. 农药学学报, 2021, 23(2): 308-315. DOI: 10.16801/j.issn.1008-7303.2021.0003
    FENG Mingxing, HU Zhaonong, WU Wenjun. Insecticidal activity of periplocosides against oriental armyworm (Mythimna separata) and its effects on three characteristic enzyme activities in the midgut cell BBMV[J]. Chinese Journal of Pesticide Science, 2021, 23(2): 308-315. DOI: 10.16801/j.issn.1008-7303.2021.0003
    Citation: FENG Mingxing, HU Zhaonong, WU Wenjun. Insecticidal activity of periplocosides against oriental armyworm (Mythimna separata) and its effects on three characteristic enzyme activities in the midgut cell BBMV[J]. Chinese Journal of Pesticide Science, 2021, 23(2): 308-315. DOI: 10.16801/j.issn.1008-7303.2021.0003

    杠柳新苷类化合物对东方黏虫的杀虫活性及对中肠细胞刷状缘膜囊泡3种特征酶活性的影响

    Insecticidal activity of periplocosides against oriental armyworm (Mythimna separata) and its effects on three characteristic enzyme activities in the midgut cell BBMV

    • 摘要: 为了研究和阐明杠柳新苷类杀虫活性化合物对东方黏虫的杀虫活性及作用靶标,采用载毒叶片饲喂法测定比较了6个杠柳新苷类化合物 (PSA、PSD、PSE、PSF、PSP和PST) 对3龄东方黏虫Mythimna separata Walker幼虫的毒力;应用MgCl2沉淀差速离心法制备东方黏虫中肠细胞刷状缘膜囊泡 (BBMV) 基础上,测定了6个化合物对东方黏虫幼虫中肠细胞BBMV中3种特征酶——氨肽酶、碱性磷酸酶及V-ATP酶活性的影响。结果表明:杠柳新苷P (PSP) 和T (PST) 对3龄东方黏虫幼虫表现出较高的杀虫活性,其24 h的致死中浓度(LC50值) 分别为1.60和1.23 mg/mL,杠柳新苷A (PSA)、D (PSD) 和F (PSF) 仅表现出微弱的杀虫活性 (LC50 > 20 mg/mL),而杠柳新苷E (PSE)则无明显杀虫活性。酶活性测定结果表明,6个杠柳新苷类化合物对氨肽酶和碱性磷酸酶活性均没有明显的抑制作用,而高杀虫活性化合物PSP和PST则可浓度依赖地抑制V-ATP酶活性。推测杠柳新苷类杀虫活性化合物的作用靶标与V-ATP酶有密切关系。

       

      Abstract: The toxicity of six periplocoside compounds against 3th M. separata larvae was measured by toxic carrying leaf disc method, the activities of aminopeptidase, alkaline phosphatase and V-ATPase in BBMV were determined using six periplocoside compounds on the basis of preparing brush border membrane vesicles (BBMV) of oriental armyworm midgut cells by MgCl2 precipitation and differential centrifugation. The results showed that PSP and PST had obvious insecticidal activity with LC50 value of 1.60 and 1.23 mg/mL at 24 h, respectively. PSA, PSD and PSF revealed weak insecticidal activity (LC50 > 20 mg/mL) while PSE had no insecticidal activity. Six periplocosides had no significant inhibitory effect on aminopeptidase and alkaline phosphatase. The activity of V-ATPase was significantly inhibited by PSP and PST with a concentration dependence manner. Therefore, we preliminarily speculated that the initial binding site of insecticidal periplocoside compounds was located on the V-ATPase.

       

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