李同宾, 李红, 孙丰收, 杨松, 李北兴, 慕卫. QuEChERS-超高效液相色谱-串联质谱法测定玉米粉中41种农药残留[J]. 农药学学报, 2021, 23(1): 154-161. DOI: 10.16801/j.issn.1008-7303.2021.0013
    引用本文: 李同宾, 李红, 孙丰收, 杨松, 李北兴, 慕卫. QuEChERS-超高效液相色谱-串联质谱法测定玉米粉中41种农药残留[J]. 农药学学报, 2021, 23(1): 154-161. DOI: 10.16801/j.issn.1008-7303.2021.0013
    LI Tongbin, LI Hong, SUN Fengshou, YANG Song, LI Beixing, MU Wei. Determination of 41 pesticides in maize meal by QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2021, 23(1): 154-161. DOI: 10.16801/j.issn.1008-7303.2021.0013
    Citation: LI Tongbin, LI Hong, SUN Fengshou, YANG Song, LI Beixing, MU Wei. Determination of 41 pesticides in maize meal by QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2021, 23(1): 154-161. DOI: 10.16801/j.issn.1008-7303.2021.0013

    QuEChERS-超高效液相色谱-串联质谱法测定玉米粉中41种农药残留

    Determination of 41 pesticides in maize meal by QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry

    • 摘要: 基于QuEChERS-超高效液相色谱-串联质谱 (UPLC-MS/MS),建立了同时检测玉米粉中41种常见农药残留的分析方法。玉米粉样品经乙腈提取、QuEChERS方法净化后,采用UPLC-MS/MS检测分析。结果表明:在0.5~100 μg/L 范围内,41种待检农药质量浓度与对应的峰面积间均呈良好线性关系,决定系数均在 0.99以上;41种农药的定量限 (LOQ) 为 0.48~0.85 μg/kg;在0.01、0.1和1 mg/kg添加水平下,41种农药的平均回收率在74%~110%之间,相对标准偏差 (RSD) 在1.5%~12% (n = 5) 之间。该方法高效、快速、灵敏、准确,适用于玉米粉中多种农药残留的同时检测。

       

      Abstract: Based on QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), a method for the simultaneous detection of 41 pesticide residues in maize meal was established. Maize meal samples were extracted with acetonitrile and analysed by UPLC-MS/MS after treated with QuEChERS approach. Good linearity was observed when the mass concentration of 41 pesticides ranged between 0.5 and 100 μg/L, with all of the determination coefficients higher than 0.99. Limit of quantitations (LOQs) of the pesticides ranged from 0.48 to 0.85 μg/kg. At the fortified levels of 0.01, 0.1, and 1 mg/kg, average recoveries of the 41 pesticides ranged between 74% and 110%, and the relative standard deviations (RSDs) were 1.5% to 12% (n = 5). This new method is efficient, fast, sensitive and accurate, which is suitable for the simultaneous detection of multiple pesticides in maize meal.

       

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