Abstract: The detection and analysis of organophosphorus pesticide metabolites play an important role in the determination of organophosphorus pesticide nosotoxicosis and poisoning cases, clinical diagnosis, biological monitoring and so on. The development of accurate detection technology of trace and even ultra trace organophosphorus pesticide metabolites, and the establishment of a method for the detection of organophosphorus pesticide metabolites in complex biological samples have become one of the urgent problems to be solved in forensic science. At present, the common laboratory detection methods at home and abroad include gas chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc. This paper focuses on the research progress of these detection technologies, the pretreatment techniques for blood and urine samples such as liquid-liquid extraction, solid-phase extraction and QuEChERS, as well as the pretreatment techniques for hair samples such as the solid-liquid extraction method. In addition, the principle and application progress of fluorescent probe, immunoassay, biosensor and other rapid detection technologies are introduced, and the problems of existing detection technologies are summarized.