吴倩倩, 庄超杰, 吴鉴艳, 张传清. 浙江省西 (甜) 瓜蔓枯病菌对甲基硫菌灵和啶酰菌胺的抗性检测及抗性机制[J]. 农药学学报, 2021, 23(6): 1117-1122. DOI: 10.16801/j.issn.1008-7303.2021.0129
    引用本文: 吴倩倩, 庄超杰, 吴鉴艳, 张传清. 浙江省西 (甜) 瓜蔓枯病菌对甲基硫菌灵和啶酰菌胺的抗性检测及抗性机制[J]. 农药学学报, 2021, 23(6): 1117-1122. DOI: 10.16801/j.issn.1008-7303.2021.0129
    WU Qianqian, ZHUANG Chaojie, WU Jianyan, ZHANG Chuanqing. Resistance monitoring and mechanism of Stagonosporppsis citrulli to thiophanate methyl and boscalid in Zhejiang Province[J]. Chinese Journal of Pesticide Science, 2021, 23(6): 1117-1122. DOI: 10.16801/j.issn.1008-7303.2021.0129
    Citation: WU Qianqian, ZHUANG Chaojie, WU Jianyan, ZHANG Chuanqing. Resistance monitoring and mechanism of Stagonosporppsis citrulli to thiophanate methyl and boscalid in Zhejiang Province[J]. Chinese Journal of Pesticide Science, 2021, 23(6): 1117-1122. DOI: 10.16801/j.issn.1008-7303.2021.0129

    浙江省西 (甜) 瓜蔓枯病菌对甲基硫菌灵和啶酰菌胺的抗性检测及抗性机制

    Resistance monitoring and mechanism of Stagonosporppsis citrulli to thiophanate methyl and boscalid in Zhejiang Province

    • 摘要: 从浙江省5地采集了112株西 (甜) 瓜蔓枯病菌Stagonosporppsis citrulli,采用区分剂量法检测其对苯并咪唑类杀菌剂甲基硫菌灵 (以下简称Ben) 和琥珀酸脱氢酶抑制剂类 (SDHIs) 杀菌剂啶酰菌胺 (简称Bos) 的抗性。结果显示:112株西 (甜) 瓜蔓枯病菌对Ben和Bos的抗药性频率分别为100%和28.6%,其中对甲基硫菌灵产生高水平抗性 (BenHR) 的菌株达100%,对啶酰菌胺产生低水平抗性 (BosLR) 和高水平抗性 (BosHR) 的菌株分别为18.8%和9.8%。抗药性分子机制研究表明:BenHR菌株中β-tubulin的第198位氨基酸由Glu (E) 突变成了Ala (A);BosHR菌株中Sdh B的第277位氨基酸由His (H) 突变成了Tyr (Y),但BosLR的抗性机制还需进一步研究。研究结果表明,浙江省西 (甜) 瓜蔓枯病菌对苯并咪唑类杀菌剂甲基硫菌灵的抗性已十分严重,尽管大多数菌株对啶酰菌胺仍表现敏感,但在一些地区已存在高水平抗性菌株,应在加强抗药性监测的同时,注意SDHIs类杀菌剂的科学使用。

       

      Abstract: A total of 112 isolates of Stagonosporppsis citrulli were collected from 5 locations in Zhejiang Province. The resistance to thiophanate-methyl (Ben) and boscalid (Bos) was detected by the method of differentiated doses. The results showed that the respective resistance frequencies were 100% and 28.6% to Ben and Bos, respectively. The high-level resistance (BenHR) of thiophanate-methyl reached 100%, and the low-level resistance (BosLR) and high-level resistance (BosHR) of boscalid were 18.8% and 9.8%, respectively. Further studies on the molecular mechanism of resistance showed that the BenHR of S. citrulli was caused by the E198A point mutation in β-tubulin, which was the mutation of 198th amino acid of from Glu (E) to Ala (A). The mechanism of BosHR was the point mutation in 277th amino acid of Sdh B from His (H) to Tyr (Y), but the mechanism of low-level resistance (BosLR) still needs to be studied. To sum up, the resistance of S. citrulli population to benzimidazole fungicides in Zhejiang Province was very serious. Most isolates were sensitive to boscalid, but the high-level resistance has occurred in some regions. Therefore, attention should be paid to the scientific use of SDHIs and resistance monitoring should be strengthened.

       

    /

    返回文章
    返回