Abstract: Botrytis cinerea is the pathogen of many crops gray mold disease and its conidia is the major carrier for disease epidemic. In this work, the carbon utilization and conidia formation of B. cinerea were analyzed by phenomic technology. In addition, the carbon sources for conidia production of this pathogen under five different fungicides (carbendazim, propiconazole, pyrimethanil, iprodione and prochloraz) were also tested. These results showed that about 92 carbon sources could be metabolized by B. cinerea, including carbohydrates, amino acids, and others. Among them, about 35 carbon sources such as amygdalin and L-arabinose could be metabolized to produce conidia. And about 57 carbon sources (tween 80, D-arabinose, glucuronic acid, etc.) could be metabolized by B. cinerea, but could not promote the conidia formation. When treated by 1 mg/L of carbendazim and 5 mg/L of prochloraz, and at both 1 and 10 mg/L of propiconazole, the carbon sources for conidia production of B. cinerea declined greatly. While treated for 0.08 and 1 mg/L of pyrimethanil or 0.1 and 5 mg/L of iprodione, they showed no inhibition. The results mentioned above revealed the carbon sources for conidia production of B. cinerea under chemical pressures of fungicides, which give us some constructive suggestions for the fungicide selection in gray mold management.