刘尚可, 王越, 潘灿平, 贾厚振, 刘铭钰, 邹楠. 外源单宁酸对茶叶中农药胁迫作用的缓解效应[J]. 农药学学报, 2021, 23(6): 1150-1158. DOI: 10.16801/j.issn.1008-7303.2021.0173
    引用本文: 刘尚可, 王越, 潘灿平, 贾厚振, 刘铭钰, 邹楠. 外源单宁酸对茶叶中农药胁迫作用的缓解效应[J]. 农药学学报, 2021, 23(6): 1150-1158. DOI: 10.16801/j.issn.1008-7303.2021.0173
    LIU Shangke, WANG Yue, PAN Canping, JIA Houzhen, LIU Mingyu, ZOU Nan. Mitigating effect of exogenous tannic acid on the stress of pesticides in tea[J]. Chinese Journal of Pesticide Science, 2021, 23(6): 1150-1158. DOI: 10.16801/j.issn.1008-7303.2021.0173
    Citation: LIU Shangke, WANG Yue, PAN Canping, JIA Houzhen, LIU Mingyu, ZOU Nan. Mitigating effect of exogenous tannic acid on the stress of pesticides in tea[J]. Chinese Journal of Pesticide Science, 2021, 23(6): 1150-1158. DOI: 10.16801/j.issn.1008-7303.2021.0173

    外源单宁酸对茶叶中农药胁迫作用的缓解效应

    Mitigating effect of exogenous tannic acid on the stress of pesticides in tea

    • 摘要: 农药是控制病虫草害的重要手段,但农药也会对植物的氧化系统及营养品质产生影响。基于单宁酸的天然属性和结构特点,研究了在农药诱导的氧化胁迫下,叶面施用单宁酸对茶叶抗氧化系统和营养品质的潜在作用。与单独施用农药相比,加施10 mg/L单宁酸显著提高了茶叶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及抗坏血酸过氧化物酶(APX)活性,其中POD活性分别是空白对照、吡唑醚菌酯单独施用的4.69倍和3.13倍,APX活性比单独施用吡虫啉、联苯菊酯和吡唑醚菌酯分别提高了340.74%、114.71%和271.70%。此外,单宁酸显著提高了茶叶总抗氧化能力,提高了脯氨酸、还原型谷胱甘肽和茶多酚含量,降低了膜脂过氧化产物丙二醛的含量。单宁酸还提高了茶叶中维生素C、可溶性蛋白、咖啡碱和游离氨基酸的含量。综上,外源单宁酸能够激活茶叶抗氧化酶活性,影响抗氧化非酶物质的积累,缓解茶叶中农药胁迫带来的氧化损伤,最终提高茶叶品质。

       

      Abstract: Pesticides are important tools to manage diseases, insect pests and weeds. And they can also affect the oxidation system and nutritional quality of plants. Based on the natural properties and structural characteristics of tannin acid, the potential effects of foliar application of tannin acid on the antioxidant system and nutritional quality of tea under oxidative stress induced by pesticides were studied. Compared with the pesticide application alone, the addition of 10 mg/L tannic acid significantly increased tea superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity, in which the POD activity is 4.69 times and 3.13 times that of the control and pyraclostrobin treatment respectively. Compared with the application of imidacloprid, bifenthrin and pyraclostrobin alone, the APX activity is increased by 340.74%, 114.71% and 271.70%. In addition, tannin acid significantly increased the total antioxidant capacity of tea and the content of proline. Tannin acid reduced glutathione, tea polyphenols, the content of malondialdehyde and membrane lipid peroxidation products. Tannic acid also increases the content of vitamin C, soluble protein, caffeine and free amino acids in tea. In summary, exogenous tannin acid can activate antioxidant enzyme activity of tea leaf, affect the accumulation of antioxidant non-enzymatic substances, alleviate the oxidative damage caused by pesticide stress in tea, and improve the quality of tea ultimately.

       

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