罗荪琳, 范瑞祺, 张婉君, 贾栗, 苑晓燕, 陈义强. 通过基准剂量法比较氟氯氰菊酯和高效氯氟氰菊酯对斑马鱼的胚胎发育毒性[J]. 农药学学报, 2022, 24(4): 844-850. DOI: 10.16801/j.issn.1008-7303.2022.0025
    引用本文: 罗荪琳, 范瑞祺, 张婉君, 贾栗, 苑晓燕, 陈义强. 通过基准剂量法比较氟氯氰菊酯和高效氯氟氰菊酯对斑马鱼的胚胎发育毒性[J]. 农药学学报, 2022, 24(4): 844-850. DOI: 10.16801/j.issn.1008-7303.2022.0025
    LUO Sunlin, FAN Ruiqi, ZHANG Wanjun, JIA Li, YUAN Xiaoyan, CHEN Yiqiang. Comparison of the embryonic developmental toxicity of cyfluthrin and lambda-cyhalothrin on zebrafish through the benchmark dose (BMD) method[J]. Chinese Journal of Pesticide Science, 2022, 24(4): 844-850. DOI: 10.16801/j.issn.1008-7303.2022.0025
    Citation: LUO Sunlin, FAN Ruiqi, ZHANG Wanjun, JIA Li, YUAN Xiaoyan, CHEN Yiqiang. Comparison of the embryonic developmental toxicity of cyfluthrin and lambda-cyhalothrin on zebrafish through the benchmark dose (BMD) method[J]. Chinese Journal of Pesticide Science, 2022, 24(4): 844-850. DOI: 10.16801/j.issn.1008-7303.2022.0025

    通过基准剂量法比较氟氯氰菊酯和高效氯氟氰菊酯对斑马鱼的胚胎发育毒性

    Comparison of the embryonic developmental toxicity of cyfluthrin and lambda-cyhalothrin on zebrafish through the benchmark dose (BMD) method

    • 摘要: 氟氯氰菊酯和高效氯氟氰菊酯是两种常见的拟除虫菊酯类杀虫剂,因对水生生物具有高毒性,其环境风险有待进一步评估。本研究以斑马鱼胚胎为模型,将其连续暴露于两种农药下96 h,期间分别测定了受精后24 h胚胎自主运动、受精后48 h仔鱼心率和孵化率、受精后96 h仔鱼畸形率和死亡率等指标,以评价两种农药对斑马鱼的胚胎发育毒性,并采用基准剂量(BMD)法分析比较了两种药剂的胚胎发育毒性敏感指标,探究了BMD法在评价农药环境风险中的应用前景。BMD法计算结果显示,评价氟氯氰菊酯毒性时最敏感的指标为受精后48 h仔鱼心率,评价高效氯氟氰菊酯毒性时最敏感的指标为受精后48 h孵化率,且氟氯氰菊酯最敏感指标的毒性阈值(基准剂量置信区间下限,BMDL10)更低。此外,针对两种农药的绝大多数胚胎发育毒性指标计算所得的BMDL10值均低于对应的最小可见损害作用水平(LOAEL) 。结果提示,BMD法不仅可用于比较相同种类不同农药的毒性差异,还能充分准确地挖掘实验数据,可为农药的环境风险评估提供新的思路和策略。

       

      Abstract: Cyfluthrin and lambda-cyhalothrin are two typical insecticides that are widely used. In this study, zebrafish (Danio rerio) embryos were used as a model and were continuously exposed to the two pesticides for 96 h. During the period, the autonomous tail flicks at 24 h post fertilization, the heart rate and hatching rate of larvae at 48 h post fertilization, and the deformity rate and mortality rate of larvae at 96 h post fertilization were measured, and such indicators were considered to evaluate the embryonic developmental toxicity of two pyrethroid insecticides. To explore the application prospect of the benchmark dose (BMD) method in evaluating the environmental risk of pesticides, the BMD method was used to calculate their sensitivity indicators. The most sensitive indicator of cyfluthrin was 48 h post-fertilization juvenile heart rate, the most sensitive indicator of lambda-cyhalothrin was 48 h post-fertilization hatching rate and the toxicity threshold of cyfluthrin was lower. In addition, most BMDL10 of different developmental parameters of cyfluthrin and beta-cyhalothrin were lower than the LOAEL values. The results suggest that the BMD method can compare the differences in the toxicity of similar pesticides and fully and accurately mine the experimental data, which can provide new ideas and strategies for the environmental risk assessment of pesticides.

       

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