• 百种中国杰出学术期刊
  • 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国高校精品科技期刊
  • 中国国际影响力优秀学术期刊
  • 中国科技核心期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状

齐永志 李海燕 苏媛 甄文超

齐永志, 李海燕, 苏媛, 甄文超. 小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状[J]. 农药学学报, 2014, 16(3): 271-280. doi: 10.3969/j.issn.1008-7303.2014.03.05
引用本文: 齐永志, 李海燕, 苏媛, 甄文超. 小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状[J]. 农药学学报, 2014, 16(3): 271-280. doi: 10.3969/j.issn.1008-7303.2014.03.05
QI Yongzhi, LI Haiyan, SU Yuan, ZHEN Wenchao. Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis[J]. Chinese Journal of Pesticide Science, 2014, 16(3): 271-280. doi: 10.3969/j.issn.1008-7303.2014.03.05
Citation: QI Yongzhi, LI Haiyan, SU Yuan, ZHEN Wenchao. Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis[J]. Chinese Journal of Pesticide Science, 2014, 16(3): 271-280. doi: 10.3969/j.issn.1008-7303.2014.03.05

小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状

doi: 10.3969/j.issn.1008-7303.2014.03.05
基金项目: 

“十二五”国家科技支撑计划课题(2011BAD16B08,2012BAD04B06,2013BAD07B05).

Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis

  • 摘要: 采用菌丝生长速率法,分别测定了采自河北、山东和河南未使用过酰胺类药剂地区的166 株小麦纹枯病菌Rhizotonia cerealis Van der Hoeven对噻呋酰胺的敏感性,并对小麦纹枯病菌抗噻呋酰胺突变体的诱导方法及突变体的主要生物学性状进行了研究。结果表明:噻呋酰胺对166株小麦纹枯病菌的平均 EC50值为(0.072 ± 0.022)μg/mL,菌株频率呈连续单峰曲线分布,未发现敏感性明显下降的亚群体,因此可将该EC50值作为小麦纹枯病菌对噻呋酰胺的敏感基线。通过紫外诱导加菌落角变的方法获得了5株抗噻呋酰胺突变体,其抗药性水平介于5.5 ~ 18.9倍之间;药剂驯化未能获得抗药性突变体。突变体在PSA平板上的生长速率与亲本菌株无显著性差异,但其菌丝干重、菌核形成数量和菌核干重均明显低于亲本菌株;除突变体HD7-7U对石新828的致病力未发生明显变化外,其他突变体对石新828和良星99的致病力均明显减弱;5株突变体的抗药性均不能稳定遗传;噻呋酰胺与戊唑醇、井冈霉素、咯菌腈、三唑酮、丙环唑和多菌灵之间无交互抗性关系。
  • BOEREMA G H, VERHOEVEN A A. Check-list for scientific names of common parasitic fungi. Series 2b: Fungi on field crops: Cereals and grasses[J]. Neth J Plant Pathol, 1977, 83(5): 165-204.
    贾廷祥, 吴桂本, 刘传德. 我国小麦根腐性病害研究现状及防治对策[J]. 中国农业科学, 1995, 28(3): 41-48. JIA Tingxiang, WU Guiben, LIU Chuande. The present research situation and control countermeasure of root rots in wheat[J]. Scientia Agric Sinica, 1995, 28(3): 41-48. (in Chinese)
    檀根甲, 季伯衡. 小麦纹枯病的研究进展[J]. 安徽农业大学学报, 1998, 25(1): 70-75. TAN Genjia, JI Boheng. Research progress of wheat sharp eyespot[J]. J Anhui Agric Univ, 1998, 25(1): 70-75. (in Chinese)
    陈健华, 张炽昌, 徐东方, 等. 小麦纹枯病的研究进展[J]. 现代农业科技, 2011(1): 169-170. CHEN Jianhua, ZHANG Chichang, XU Dongfang, et al. Research progress of wheat sharp eyespot[J]. Modern Agric Sci Tech, 2011(1): 169-170. (in Chinese)
    王金凤, 杜丽璞, 李钊, 等. 抗纹枯病、根腐病的转SN1基因小麦的获得与鉴定[J]. 作物学报, 2012, 38(5): 773-779. WANG Jinfeng, DU Lipu, LI Zhao, et al. Development and characterization of SN1 transgenic wheat plants with enhanced resistance to Rhizoctonia cerealis and Bipolaris sorokiniana[J]. Acta Agronomica Sinica, 2012, 38(5): 773-779. (in Chinese)
    刘朝晖, 张旭, 陆维忠. 小麦纹枯病的研究进展和对策[J]. 江苏农业学报, 2000, 16(3): 185-190. LIU Chaohui, ZHANG Xu, LU Weizhong. Research advances in wheat sharp eyespot and further control strategies[J]. Jiangsu J Agric Sci, 2000, 16(3): 185-190. (in Chinese)
    王怀训, 王开运, 蔡春菊, 等. 立克秀防治小麦纹枯病的药效评价[J]. 农药, 2000, 39(12): 31-32. WANG Huaixun, WANG Kaiyun, CAI Chunju, et al. The evaluation of tebuconazole control wheat sharp eyespot[J]. Pesticides, 2000, 39(12): 31-32. (in Chinese)
    李山东, 于金凤, 彭迪, 等. 枯草芽孢杆菌NJ18和氟酰胺联合拌种防治小麦纹枯病研究[J]. 农药学学报, 2013, 15(4): 427-433. LI Shandong, YU Jinfeng, PENG Di, et al. Co-utilization of Bacillus subtilis and flutolanil in controlling sharp eyespot of wheat by seed dressing[J]. Chin J Pestic Sci, 2013, 15(4): 427-433. (in Chinese)
    胡燕, 王怀训, 夏晓明, 等. 四地区小麦纹枯病菌对6种杀菌剂的抗性比较[J]. 植物保护学报, 2006, 33(4): 423-427. HU Yan, WANG Huaixun, XIA Xiaoming, et al. Susceptibility of Rhizoctonia cerealis to six fungicides in four wheat areas[J]. Acta Phytophylacica Sinica, 2006, 33(4): 423-427. (in Chinese)
    PRASANNA KUMAR M K, SIDDE GOWDA D K, PANDURANGE GOWDA K T, et al. A new carboxynilide group fungicide against paddy sheath blight[J]. Res J Agric Sci, 2012, 3(2): 500-505.
    吉沐祥, 陈宏州, 吴祥, 等. 噻呋酰胺与氟环唑及其混配对水稻纹枯病菌抑菌作用[J]. 农学学报, 2014, 4(1): 15-18. JI Muxiang, CHEN Hongzhou, WU Xiang, et al. The bacteriostatic action of thifluzamide, epoxiconazole and their mixtures on rice sheath blight[J]. J Agric, 2014, 4(1): 15-18. (in Chinese)
    孙春来, 陈怀谷, 吉用铨, 等. 噻呋酰胺等杀菌剂防治小麦纹枯病田间试验[J]. 现代农药, 2012, 11(6): 42-44. SUN Chunlai, CHEN Huaigu, JI Yongquan, et al. Field trials of seven fungicides including thifluzamide against wheat sharp eyespot[J]. Modern Agrochem, 2012, 11(6): 42-44. (in Chinese)
    陈香华, 赵桂东, 李茹, 等. 不同药剂处理对小麦纹枯病防治效果的研究[J]. 金陵科技学院学报, 2012, 28(4): 50-52. CHEN Xianghua, ZHAO Guidong, LI Ru, et al. The control effect of different medicament treatment on wheat sharp eyespot[J]. J Jinling Inst Technol, 2012, 28(4): 50-52. (in Chinese)
    关立松. 240 g/L满穗悬浮剂防治小麦纹枯病药效研究[J]. 现代农业科技, 2013(8): 94, 99. GUAN Lisong. Study on the control effect of 240 g/L thifluzamide SC on wheat sharp eyespot[J]. Modern Agric Sci Tech, 2013(8): 94, 99. (in Chinese)
    GORGOLOUS S G, DEKKER J. Detection and measurement of fungicide resistance general principles[J]. FAO Plant Prot Bull, 1982, 30(2): 39-71.
    MENG Qingxiao, CUI Xiaolan, BI Yang, et al. Study on the resistance risk and resistance inheritance of Phytophthora capsici to flumorph[J]. Phytopathology, 2008, 98(Suppl.): 128.
    赵卫松, 韩秀英, 齐永志, 等. 辣椒疫霉对双炔酰菌胺的敏感性测定及其抗药突变体生物学性状研究[J]. 农药学学报, 2011, 13(1): 21-27. ZHAO Weisong, HAN Xiuying, QI Yongzhi, et al. Study on the sensitivity of Phytophthora capsici to mandipropamid and biological characteristics of resistant mutants[J]. Chin J Pestic Sci, 2011, 13(1): 21-27. (in Chinese)
    崔晓岚, 孟庆晓, 毕扬, 等. 辣椒疫霉对烯酰吗啉的敏感性基线及室内抗药突变体研究[J]. 植物病理学报, 2009, 39(6): 630-637. CUI Xiaolan, MENG Qingxiao, BI Yang, et al. Baseline sensitivity and laboratory mutants of Phytophthora capsici resistant to dimethomorph[J]. Acta Phytopathologica Sinica, 2009, 39(6): 630-637. (in Chinese)
    王育水. 环境因素对禾谷丝核菌菌核形成的影响[J]. 麦类作物学报, 2008, 28(1): 160-164. WANG Yushui. Influences of environmental factors on the Sclerotium formation of Rhizoctonia cerealis Vander Hoeven[J]. J Triticeae Crops, 2008, 28(1): 160-164. (in Chinese)
    CHEN Y, ZHANG A F, WANG W X, et al. Baseline sensitivity and efficacy of thifluzamide in Rhizoctonia solani[J]. Ann Appl Bio, 2012, 161(3): 247-254.
    刘英华. 小麦纹枯病菌抗戊唑醇菌系生物学特性及其抗性机理研究 [D]. 泰安: 山东农业大学, 2003. LIU Yinghua. Studies on biological characters and resistance mechanism of Rhizoctonia cerrealis to tebuconazole [D]. Tai'an: Shangdong Agricultural University, 2003. (in Chinese)
    汪敏, 吕柏林, 邢小萍, 等. 河南省小麦纹枯病菌的群体组成及其致病力分化研究[J]. 植物病理学报, 2011, 41(5): 556-560. WANG Min, LV Bolin, XING Xiaoping, et al. Composition and virulence variation of the pathogen of wheat sharp eyespot from Henan province[J]. Acta Phytopathologica Sinica, 2011, 41(5): 556-560. (in Chinese)
    WONG F P, WILCOX W F. Sensitivity to azoxystrobin among isolates of Uncinula necator: baseline distribution and relationship to myclobutanil sensitivity[J]. Plant Dis, 2002, 86(4): 394-404.
    夏晓明. 禾谷丝核菌(Rhizoctonia cerrealis)对戊唑醇的抗性机制研究 [D]. 泰安: 山东农业大学, 2006. XIA Xiaoming. Studies on the resistance mechnaism of Rhizoctonia cerrealis to tebuconazole [D]. Tai'an: Shangdong Agricultural University, 2006. (in Chinese)
    张骞, 周明国, 叶钟音. 植物病原真菌对甲基立枯磷的抗药性及风险研究[J]. 农药学学报, 2000, 2(2): 22-28. ZHANG Qian, ZHOU Mingguo, YE Zhongyin. Resistance risk of plant pathogenous fungi to tolclofos-methyl[J]. Chin J Pestic Sci, 2000, 2(2): 22-28. (in Chinese)
    KEON J P R, WHITE G A, HARGREAVES J A. Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis[J]. Curr Genet, 1991, 19(6): 475-481.
    SHIMA Y, ITO Y, KANEKO S, et al. Identification of three mutant loci conferring carboxin-resistance and development of a novel transformation system in Aspergillus oryzae[J]. Fungal Genet Biol, 2009, 46(1): 67-76.
    WEBER R W S. Resistance of Botrytis cinerea to multiple fungicides in northern German small-fruit production[J]. Plant Dis, 2011, 95(10): 1263-1269.
    WHARTON P, FAIRCHILD K, BELCHER A, et al. First report of in-vitro boscalid-resistant isolates of Alternaria solani causing early blight of potato in Idaho[J]. Plant Dis, 2012, 96(3): 454.
    CHEN F, LIU X, CHEN S, et al. Characterization of Monilinia fructicola strains resistant to both propiconazole and boscalid[J]. Plant Dis, 2013, 97(5): 645-651.
    李良孔. 黄瓜白粉病菌对氟吡菌酰胺敏感基线的建立及其抗药性风险评估 [D]. 长春: 吉林大学, 2011. LI Liangkong. Baseline sensitivity of Podosphaera xanthii to fluopyram and its resistance risk assessment [D]. Changchun: Jilin University, 2011. (in Chinese)
    AVENOT H F, MICHAILIDES I T J. Progress in understanding molecular mechanisms and evolution of resistance to succinate dehydrogenase inhibiting (SDHI) fungicides in phytopathogenic fungi[J]. Crop Prot, 2010, 29: 643-651.
    刘西莉, 牟文君, 李波涛, 等. 一种鉴定水稻纹枯病菌Sdh基因核苷酸点突变及其对噻呋酰胺抗药性的方法: 中国,201210281520.5 [P]. 2013-01-09. LIU Xili, MOU Wenjun, LI Botao, et al. A method for identification on Sdh nucleotide point mutation of Rhizoctonia solani and its resistance to trifluzamide: China,201210281520.5 [P]. 2013-01-09. (in Chinese)
  • 加载中
计量
  • 文章访问数:  3867
  • HTML全文浏览量:  594
  • PDF下载量:  755
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-02-12
  • 修回日期:  2014-03-11
  • 刊出日期:  2014-06-14

目录

    /

    返回文章
    返回