小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状

齐永志; 李海燕; 苏媛; 甄文超

doi:10.3969/j.issn.1008-7303.2014.03.05

小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状

doi: 10.3969/j.issn.1008-7303.2014.03.05

1.
河北农业大学植物保护学院, 河北保定 071001

基金项目:

“十二五”国家科技支撑计划课题（2011BAD16B08，2012BAD04B06，2013BAD07B05）.

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出版历程
- 收稿日期: 2014-02-12
- 修回日期: 2014-03-11
- 刊出日期: 2014-06-14

Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis

1.
College of Plant Protection, Agricultural University of Hebei, Baoding 071001, Hebei Province, China

摘要

摘要: 采用菌丝生长速率法，分别测定了采自河北、山东和河南未使用过酰胺类药剂地区的166 株小麦纹枯病菌Rhizotonia cerealis Van der Hoeven对噻呋酰胺的敏感性，并对小麦纹枯病菌抗噻呋酰胺突变体的诱导方法及突变体的主要生物学性状进行了研究。结果表明：噻呋酰胺对166株小麦纹枯病菌的平均 EC50值为（0.072 ± 0.022）μg/mL，菌株频率呈连续单峰曲线分布，未发现敏感性明显下降的亚群体，因此可将该EC50值作为小麦纹枯病菌对噻呋酰胺的敏感基线。通过紫外诱导加菌落角变的方法获得了5株抗噻呋酰胺突变体，其抗药性水平介于5.5 ~ 18.9倍之间；药剂驯化未能获得抗药性突变体。突变体在PSA平板上的生长速率与亲本菌株无显著性差异，但其菌丝干重、菌核形成数量和菌核干重均明显低于亲本菌株；除突变体HD7-7U对石新828的致病力未发生明显变化外，其他突变体对石新828和良星99的致病力均明显减弱；5株突变体的抗药性均不能稳定遗传；噻呋酰胺与戊唑醇、井冈霉素、咯菌腈、三唑酮、丙环唑和多菌灵之间无交互抗性关系。
- 小麦纹枯病菌 /
- 噻呋酰胺 /
- 敏感性 /
- 抗药性突变体 /
- 生物学性状
Abstract: The sensitivity of 166 isolates of R. cerealis to trifluzamide collected from areas in Hebei, Henan, and Shandong provinces where no novel amides fungicides had been used before were determined by mycelial growth rate method. UV mutagenesis and fungicide adaptation test were conducted to acquire resistant mutants to trifluzamide and to clarify the biological characteristics of the resistant mutants. The results showed that the frequency of isolates with different sensitivity to trifluzamide distributed as a unimodal curve, and there was no sub-group with declining sensitivity. The mean EC50 value was 0.072±0.022 μg/mL and it could be considered as the sensitivity baseline. Five resistant mutants of R. cerealis to trifluzamide were obtained by UV mutagenesis with the resistance levels ranging from 5.5~18.9 fold. There was no significant difference on the growth rate of mycelium on PSA between the mutants and the parent isolates, but the dry weight of mycelium, the number of sclerotium and the dry weight of sclerotium were significantly lower than that of the parent isolates. The virulence of five resistant mutants were significantly weakened to Shixin828 and Liangxing 99 than that of the parent isolates, except for the virulence of resistant mutant HD7-7U to Shixin 828, and the resistance of five mutants could not be inherited stably. No cross-resistance existed between trifluzamide and triadimefon, jinggangmycin, tebuconazole, fludioxonil, propiconazole and carbendazim.
- Rhizoctonia cerealis /
- trifluzamide /
- sensitivity /
- resistant mutants /
- biological characteristics

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