CUI Guangrui, ZHANG Kai, GUAN Zewei, LI Jiaran, LIU Hui, WEN Xun, CAI Meng, YANG Guangfu. Inhibitory activity of enprocymid against Fusarium graminearum and Fusarium pseudohyphomycetes and screening of its compound formulations[J]. Chinese Journal of Pesticide Science, 2024, 26(6): 1105-1116. DOI: 10.16801/j.issn.1008-7303.2024.0076
    Citation: CUI Guangrui, ZHANG Kai, GUAN Zewei, LI Jiaran, LIU Hui, WEN Xun, CAI Meng, YANG Guangfu. Inhibitory activity of enprocymid against Fusarium graminearum and Fusarium pseudohyphomycetes and screening of its compound formulations[J]. Chinese Journal of Pesticide Science, 2024, 26(6): 1105-1116. DOI: 10.16801/j.issn.1008-7303.2024.0076

    Inhibitory activity of enprocymid against Fusarium graminearum and Fusarium pseudohyphomycetes and screening of its compound formulations

    • Succinate dehydrogenase inhibitors (SDHIs) are a crucial class of broad-spectrum fungicides in the control of plant diseases. However, the majority of SDHIs exhibit limited efficacy against Fusarium head blight and crown rot in wheat. Enprocymid is a novel SDHI fungicide co-developed by Central China Normal University and Jiangsu Zhongqi Technology Co., Ltd. It is characterized by high fungicidal activity, a broad spectrum action, and low environmental and ecological risks. This study systematically investigated the potential of enprocymid via enzyme kinetic studies, molecular dynamics simulations, and evaluation of fungicidal effects both in the laboratory and in the field. The results indicated that enprocymid was a substrate-competitive SDH inhibitor with Ki of (14.86 ± 0.80) nmol/L against yeast SDH. For mycelium growth inhibition, the EC50 values of enprocymid against F. graminearum and F. pseudograminearum were 0.014 μg/mL and 0.064 μg/mL, respectively, comparable to metconazole (0.011 μg/mL and 0.026 μg/mL) and significantly superior to prothioconazole (1.795 μg/mL and 0.203 μg/mL). Enprocymid exhibited significant synergistic effects against F. pseudograminearum in combination with metconazole, with a co-toxicity coefficient (CTC) ranging from 212.26 to 757.14. The most notable synergistic effect was observed in the mixture of enprocymid and prothioconazole at a 1∶5 ratio against both F. graminearum and F. pseudograminearum, with CTC values of 290.60 and 251.45, respectively. Field trials showed that 150 g/hm2 and 180 g/hm2 of enprocymid provided an average control efficacy of 85.79% and 91.95% against wheat Fusarium head blight, and significantly reduced the toxin levels. This efficacy was comparable to that of pydiflumetofen at 150 g/hm2 (86.73%) and significantly superior to that of prothioconazole at 180 g/hm2 (82.47%). This study provides important insights into the development of enprocymid compound formulations and their field application.
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