Synthesis of Artificial Antigen and Preparation of Monoclonal Antibody for Acetochlor

The hapten of acetochlor-MPA(AMPA) was synthesized by the reaction of acetochlor with 3-mercaptopropionic acid in alkaline solution.The artificial antigens (AMAP-BSA/OVA) were synthesized by coupling of molecules between hapten and BSA,OVA respectively with active ester method.The molar ratio was 41,26 for AMPA-BSA and AMPA-OVA respectively detected by UV spectro-photometer.Mice were immunized by injection of antigen AMPA-BSA.The monoclonal antibody was produced with titer of 1∶ 16×104.The ELISA method based upon AMPA-OVA and monoclonal antibody was established.This ELISA method showed its IC50 value was 0.55 μg/ L,the response range for acetochlor was 0.04~5 μg/L,and limit of detection was 0.04 μg/L.