Abstract: A method for the determination of benziothiazolinone in vegetable and fruit was developed using QuEChERS-liquid chromatography-tandem mass spectrometry. The sample was extracted with acidic acetonitrile, and then cleaned up by PEP and C18. The analyte was separated on C18 column by gradient elution with methanol-0.1% formic acid in water (including 2 mmol/mL ammonium acetate) as the mobile phases. Benziothiazolinone was determined by electrospray positive ion source (ESI+), and multiple reaction monitoring mode (MRM). The qualitative analysis was based on the retention times and characteristic ion pairs consisting of one parent ion and two fragment ions, and the quantitative analysis was carried out by the matrix-matched external standard method. The results showed that calibration curves had good linearity for benziothiazolinone and the determination coefficients (R2) were larger than 0.996; the limits of quantification (LOQs, S/N≥10) of benziothiazolinone in six different matrix (cucumber, grape, raisin, tomato sauce, tomato, apple) were10.0 μg/kg. At spiked three levels of 10, 20, 100 μg/kg in negative samples, the recoveries of benziothiazolinone were in the range of 80%-98% and the RSDs were in the range of 2.4%-13%. This novel method is highly effective, and the sensitivity, accuracy and precision are suitable for the monitoring of pesticide residues.