Abstract: In this study, 12 compounds were isolated from the methanol extract of Wikstroemia chamedaphne Meisn. using a variety of separation techniques. Based on the NMR, MS and the data in literatures, they were identified as 1-hexanoic acid-2,3-stearic acid triglyceride ( 1 ), β-sitosterol ( 2 ), dodecanol ( 3 ), cis-octadeca-9-enoic acid( 4 ), (2E)-heptyl-3-(3,4-dihydroxyphenyl) acrylate ( 5 ), (–)-pluviatolide ( 6 ), 1β-hydroxy-10β-H-guaia-4,11-dien-3-one ( 7 ), isochamaejasmin ( 8 ), neochamaejasmin B ( 9 ), stelleranol ( 10 ), glyceryl monopalmitate ( 11 ) and (+)-nortrachelogenin ( 12 ). Among them, compounds 3 , 4 and 6-12 were isolated from this plant for the first time. The results of antifungal activity evaluation indicated that isochamaejasmin ( 8 ) and neochamaejasmin B ( 9 ) showed significant inhibitory effects on the mycelial growth and the spore germination of Phytophthora infestans. The EC₅₀ values of 8 and 9 were 68.4 μg/mL and 44.9 μg/mL for the growth of P. infestans, respectively. And the EC₅₀ values of 8 and 9 were 25.1 μg/mL and 14.0 μg/mL, respectively, for the spore germination of P. infestans.