Abstract: Seventy-two Curvularia coicis isolates from 4 regions in South Fujian Province were tested for their sensitivity to pyraclostrobin using mycelial growth method. And the efficacy of pyraclostrobin for the control of Coix leaf blight (CLB) was evaluated under greenhouse conditions. The results indicated that the tested isolates were sensitive to pyraclostrobin. And the EC₅₀ values of these isolates to pyraclostrobin ranged from  1.11 μg/mL to 5.06 μg/mL, with the mean value of (3.44 ± 0.98) μg/mL. The frequency curve of pyraclostrobin was continuous and unimodal, and following the normal distribution. Hence, the mean EC₅₀ value of (3.44 ± 0.98) μg/mL can be recognized as the baseline sensitivity of C. coicis to pyraclostrobin. Results of pot experiments showed that the spray of 25% pyraclostrobin EC at 250 μg a.i./mL exhibited high efficacy for the control of CLB, with the control efficacy ranging from 78.2% to 79.7%. This efficacy was as good as that resulted from the spray of 50% iprodione SC at 500 μg a.i./mL, and significantly higher than the efficacy of reference fungicide (80% mancozeb WP at 800 μg a.i./mL). This study will guide the reasonable selection of fungicides for effective management of CLB in the future.