虞婕, 许杭杰, 赵美蓉, 岳思青. 抑霉唑的毛细管电泳手性拆分及其在线富集[J]. 农药学学报, 2019, 21(3): 309-314. DOI: 10.16801/j.issn.1008-7303.2019.0048
    引用本文: 虞婕, 许杭杰, 赵美蓉, 岳思青. 抑霉唑的毛细管电泳手性拆分及其在线富集[J]. 农药学学报, 2019, 21(3): 309-314. DOI: 10.16801/j.issn.1008-7303.2019.0048
    YU Jie, XU Hangjie, ZHAO Meirong, YUE Siqing. Enantioseparation and on-column preconcentrstion of imazalil by capillary electrophoresis[J]. Chinese Journal of Pesticide Science, 2019, 21(3): 309-314. DOI: 10.16801/j.issn.1008-7303.2019.0048
    Citation: YU Jie, XU Hangjie, ZHAO Meirong, YUE Siqing. Enantioseparation and on-column preconcentrstion of imazalil by capillary electrophoresis[J]. Chinese Journal of Pesticide Science, 2019, 21(3): 309-314. DOI: 10.16801/j.issn.1008-7303.2019.0048

    抑霉唑的毛细管电泳手性拆分及其在线富集

    Enantioseparation and on-column preconcentrstion of imazalil by capillary electrophoresis

    • 摘要: 采用毛细管电泳法对抑霉唑进行手性拆分,研究了手性选择剂β-环糊精 (β-CD)、有机添加剂、NaH2PO4、NH4H2PO4和分离电压对手性拆分的影响;同时,采用堆积法对抑霉唑进行在线富集,研究了进样压力和进样时间对在线富集的影响。结果表明:在分离电压为20 kV、β-CD浓度为5 mmol/L及缓冲体系为2.5% 异丙醇 + 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4时,抑霉唑获得最佳分离度,分离度可达3.0;在分离电压为20 kV、β-CD浓度为5 mmol/L、缓冲体系为75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 + 50 mmol/L H3PO4及进样条件为13.8 kPa × 99.9 s时获得最高富集倍数,富集倍数达91~92倍。

       

      Abstract: The enantioseparation of imazalil was conducted by capillary electrophoresis, and the effects of chiral selector β-cyclodextrin(β-CD), organic additives, NaH2PO4, NH4H2PO4 and separation voltage on the resolution were studied. And the effects of the injection pressure and preconcentration times on the on-column preconcentration of imazalil were studied by the stacking method. The results showed that the best resolution was achieved under the following conditions: separation voltage 20 kV, β-CD 5 mmol/L and a buffer system of 2.5 % isopropanol + 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 . And the resolution was up to 3.0. The highest enrichment factor was obtained at the separation voltage of 20 kV using 5 mmol/L β-CD, a buffer system of 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 + 50 mmol/L H3PO4, and the injection conditions of 13.8 kPa×99.9 s. And the enrichment factor was up to 91-92 times.

       

    /

    返回文章
    返回