叶生伟, 胡嘉隽, 胡子龙, 赵龙, 郝楠, 田向荣. 硬毛棘豆根的抑菌活性成分研究[J]. 农药学学报, 2022, 24(2): 289-297. DOI: 10.16801/j.issn.1008-7303.2021.0158
    引用本文: 叶生伟, 胡嘉隽, 胡子龙, 赵龙, 郝楠, 田向荣. 硬毛棘豆根的抑菌活性成分研究[J]. 农药学学报, 2022, 24(2): 289-297. DOI: 10.16801/j.issn.1008-7303.2021.0158
    YE Shengwei, HU Jiajun, HU Zilong, ZHAO Long, HAO Nan, TIAN Xiangrong. Studies on the antimicrobial constituents from the roots of Oxytropis hirta Bunge[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 289-297. DOI: 10.16801/j.issn.1008-7303.2021.0158
    Citation: YE Shengwei, HU Jiajun, HU Zilong, ZHAO Long, HAO Nan, TIAN Xiangrong. Studies on the antimicrobial constituents from the roots of Oxytropis hirta Bunge[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 289-297. DOI: 10.16801/j.issn.1008-7303.2021.0158

    硬毛棘豆根的抑菌活性成分研究

    Studies on the antimicrobial constituents from the roots of Oxytropis hirta Bunge

    • 摘要: 为明确硬毛棘豆Oxytropis hirta Bunge根的农用抑菌活性成分,用75%乙醇浸提硬毛棘豆根,得到粗提物;再分别以石油醚、乙酸乙酯与正丁醇为溶剂对粗提物进行萃取,得到不同溶剂萃取相;通过活性追踪法对抑菌活性好的乙酸乙酯相运用现代柱层析与波谱学技术进行了分离与结构鉴定,并对所分离的化合物进行了抑菌活性评价。结果表明:硬毛棘豆根乙酸乙酯相抑菌活性最好,在质量浓度为1 × 103 mg/L时对水稻纹枯病菌Thanatephorus cucumeris和番茄灰霉病菌Botrytis cinerea的抑制率分别为57.2%和55.4%,对烟草青枯病菌Pseudomonas solanacearum与猕猴桃溃疡病菌Pseudomonas syringae pv. actinidae的抑菌圈分别为21.8 mm和19.0 mm。从乙酸乙酯相分离鉴定出6个化合物,分别为β-香树脂醇 ( 1 )、3-oxo-azukisapogenol ( 2 )、5α-豆甾-9(11)-烯-3β-醇 ( 3 )、豆甾-4-烯-3,6-二酮 ( 4 )、3β,22β,24-三羟基齐墩果-12-烯 ( 5 )和azukisapogenol ( 6 )。抑菌活性测试结果表明:化合物 1 对金黄色葡萄球菌Staphylococcus aureus的最低抑菌浓度 (MIC)为50 mg/L,对烟草青枯病菌、猕猴桃溃疡病菌和枯草芽孢杆菌Bacilus subtilis的MIC为100 mg/L;化合物 6 对水稻纹枯病菌和番茄灰霉病菌的EC50值分别为117.4mg/L和86.2 mg/L。硬毛棘豆根乙酸乙酯相对供试植物病原真菌和细菌有较好的抑制活性,其中三萜类是其主要活性成分之一,具备进一步开发为杀菌剂的潜力。

       

      Abstract: The antifungal and antibacterial chemical constituents in agriculture were identified from the roots of Oxytropis hirta Bunge. The 75% ethanol extract from the roots of O. hirta was further extracted by petroleum ether, ethy acetate and n-butanol, respectively. The most active ethy acetate phase was isolated and identifed for its chemcial constituents by mordern column chromatography and spectroscopy techniques, and the antimicrobial activity of the isolated compounds was evaluted. The results showed that the ethyl acetate phase had the best antimicrobial activity. It showed antifugal activities against Thanatephorus cucumeris and Botrytis cinerea with inhibitory rates of 57.2% and 55.4% at 1 × 103 mg/L, respectively. It also showed antibacterial activities against Pseudomonas solanacearum and Pseudomonas syringae pv. actinidae with the antibacterial circle diameter of 21.8 mm and 19.0 mm, respectively. Six compounds were isolated and identified from the ethyl acetate phase of O. hirta as β-amyrin ( 1 ), 3-oxo-azukisapogenol ( 2 ), 5α-stigmast-9(11)-en-3β-ol ( 3 ), stigmast-4-ene-3,6-dione ( 4 ), 3β,22β,24-trihydroxy-olean-12-ene ( 5 ) and azukisapogenol ( 6 ). The results of antibacterial activity showed that the minimum inhibitory concentration (MIC) of compound 1 against Staphylococcus aureus was 50 mg/L, and the MIC of compound 1 against P. solanacearum, P. syringae and Bacilus subtilis was 100 mg/L. The EC50 value of compound 6 against T. cucumeris and B. cinerea were 117.4 mg/L and 86.2 mg/L, respectively. The ethyl acetate phase of O. hirta showed better inhibitory activity against pathogenic fungi and bacteria. Triterpenoids are one of the main active constituents and have the potential to be further developed as fungicides.

       

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