毕瑞锋, 邓锁成, 付萌, 张伟伟, 张义霞. 改进的QuEChERS-高效液相色谱-串联质谱法同时测定蔬菜水果中百菌清及其代谢物残留[J]. 农药学学报, 2022, 24(2): 429-434. DOI: 10.16801/j.issn.1008-7303.2021.0161
    引用本文: 毕瑞锋, 邓锁成, 付萌, 张伟伟, 张义霞. 改进的QuEChERS-高效液相色谱-串联质谱法同时测定蔬菜水果中百菌清及其代谢物残留[J]. 农药学学报, 2022, 24(2): 429-434. DOI: 10.16801/j.issn.1008-7303.2021.0161
    BI Ruifeng, DENG Suocheng, FU Meng, ZHANG Weiwei, ZHANG Yixia. Simultaneous determination of chlorothalonil and its metabolite in fruits and vegetables using modified QuEChERS-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 429-434. DOI: 10.16801/j.issn.1008-7303.2021.0161
    Citation: BI Ruifeng, DENG Suocheng, FU Meng, ZHANG Weiwei, ZHANG Yixia. Simultaneous determination of chlorothalonil and its metabolite in fruits and vegetables using modified QuEChERS-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 429-434. DOI: 10.16801/j.issn.1008-7303.2021.0161

    改进的QuEChERS-高效液相色谱-串联质谱法同时测定蔬菜水果中百菌清及其代谢物残留

    Simultaneous determination of chlorothalonil and its metabolite in fruits and vegetables using modified QuEChERS-high performance liquid chromatography-tandem mass spectrometry

    • 摘要: 建立了同时检测蔬菜水果中百菌清及其代谢物4-羟基百菌清的高效液相色谱-串联质谱分析方法。样品经酸化乙腈提取后,采用配备大气压化学电离源的三重四极杆串联质谱仪测定。结果表明:百菌清及其代谢物4-羟基百菌清在0.005~0.2 mg/L范围内峰面积与质量浓度的线性关系良好,决定系数(R2)大于0.99;方法定量限为0.01 mg/kg。在0.01、0.05和0.2 mg/kg 3个添加水平下,百菌清及其代谢物4-羟基百菌清在结球甘蓝、黄瓜、葡萄和苹果4种基质空白样品中的平均回收率为83%~104%,相对标准偏差(RSD)为 0.73%~7.7% (n=6)。该方法简单快速、准确可靠,适合蔬菜水果中百菌清及其代谢物4-羟基百菌清的定量测定。

       

      Abstract: An analytical method for simultaneous determination of chlorothalonil and its metabolite chlorothalonil-4-hydroxy in fruits and vegetables was developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted by acidified acetonitrile, and the extraction solution was analyzed by HPLC-MS/MS equipped with APCI ion source. The results showed that good linearity was obtained in the range of 0.005-0.2 mg/L with correlation coefficients (R2) greater than 0.99. The limits of quantification were 0.01 mg/kg. At the spiked levels of 0.01, 0.05, and 0.2 mg/kg, the average recoveries in four sample matrices (cabbage, cucumber, grape, and apple) were 83%-104% with the relative standard deviations (RSDs) varying from 0.73% to 7.7% (n=6). The proposed method is simple, rapid, accurate and reliable, and it is suitable for the quantitative determination of chlorothalonil and its metabolite in fruits and vegetables.

       

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