Abstract:
In order to study the secondary metabolites produced by the mixed fermentation of marine fungi
Trichoderma harzianum ABC19819 and
Penicillium brefeldianum ABC190807, silica gel, Sephadex LH-20 gel and thin-layer chromatography were used to isolate and purify the secondary metabolites produced by the mixed fermentation. The structures of the compounds were identified by
1H NMR,
13C NMR, ESI-MS and compared with the data in the literatures. The activities of the compounds were evaluated using the third instar larvae of
Aedes aegypti. The results showed that six known compounds were isolated from the mixed culture fermentation products of the two strains and were identified as Agathic acid (
1 ), 4-hydroxybenzaldehyde (
2 ), nafuredin (
3 ), 4-hydroxy-3,6-dimethyl-2
H-pyranone (
4 ). Mevalonolactone (
5 ) and brefeldin A (
6 ), of which compound
1 was isolated for the first time from the mixed culture fermentation products of the two strains. Compounds
1 and
3 showed significant insecticidal activity against the third instar larvae of
A. aegypti, with LC
50 values of 162 mg/L and 240 mg/L after 72 h of treatment. This study is of great value for obtaining compounds with insecticidal activities through mixed fermentation.