Abstract: RNA interference (RNAi) is a highly conserved gene silencing phenomenon in eukaryotes, which has shown broad potential application in medicine and plant protection. Recently, RNAi-related products have been or about to been in the pharmaceutical and pesticide markets. For RNAi-based fungicides, although researchers have made a lot of efforts in the development of its products, the commercial application of RNAi against plant pathogens has not arrived on the market yet. Here, the development of RNAi research since 1990 is summarized, and new ideas that may help improve the development of RNAi-based fungicides are introduced. At the same time, the influence of RNAi-based fungicides on traditional chemical fungicides is discussed, which is the innovation and application of RNAi fungicides.