Abstract: Pesticides have a significant effect in controlling pests and diseases and improving the output and quality of agricultural products. However, some pesticides have been banned or restricted in China due to their high toxicity, carcinogenicity, teratogenicity, mutagenicity, and slow metabolism, which may cause the adverse effects on human beings and the environment. Organochlorine pesticides are the largest in production and consumption of the banned and restricted pesticides, which have been raised concerns for their potentially high health or environmental risks. The establishment of efficient and convenient detection methods for the banned and restricted pesticides of organochlorine residues is of great significance to ensure food safety, promote the development of green ecological agriculture, and break the technical trade barriers in food imports and exports. Immunoassay has been widely used in the detection of pesticide residues in food for its advantages of rapidity, simplicity and sensitivity. In this paper, the research progress of immunoassay methods for the banned and restricted organochlorine pesticides is reviewed, the synthesis of haptens and the preparation of antibodies are introduced, and the application of the antibodies in immunoassay (mainly including enzyme-linked immunoassay, immunochromatographic test strip, chemiluminescence immunoassay, fluorescence immunoassay and immunosensor) is summarized. It provides a reference for research on immunoassay for the banned and restricted organochlorine pesticides in the future.