段云, 关妮, 马晨, 陈棠钻, 罗金辉, 魏静. 高效液相色谱-串联质谱法测定13种甲氧基丙烯酸酯和三唑类杀菌剂在杧果中的残留及膳食摄入风险评估[J]. 农药学学报, 2023, 25(1): 175-183. DOI: 10.16801/j.issn.1008-7303.2022.0146
    引用本文: 段云, 关妮, 马晨, 陈棠钻, 罗金辉, 魏静. 高效液相色谱-串联质谱法测定13种甲氧基丙烯酸酯和三唑类杀菌剂在杧果中的残留及膳食摄入风险评估[J]. 农药学学报, 2023, 25(1): 175-183. DOI: 10.16801/j.issn.1008-7303.2022.0146
    DUAN Yun, GUAN Ni, MA Chen, CHEN Tangzuan, LUO Jinhui, WEI Jing. Dietary risk assessment of 13 strobilurin and triazole fungicide residues in mangoes by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2023, 25(1): 175-183. DOI: 10.16801/j.issn.1008-7303.2022.0146
    Citation: DUAN Yun, GUAN Ni, MA Chen, CHEN Tangzuan, LUO Jinhui, WEI Jing. Dietary risk assessment of 13 strobilurin and triazole fungicide residues in mangoes by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2023, 25(1): 175-183. DOI: 10.16801/j.issn.1008-7303.2022.0146

    高效液相色谱-串联质谱法测定13种甲氧基丙烯酸酯和三唑类杀菌剂在杧果中的残留及膳食摄入风险评估

    Dietary risk assessment of 13 strobilurin and triazole fungicide residues in mangoes by high performance liquid chromatography-tandem mass spectrometry

    • 摘要: 为掌握我国杧果中甲氧基丙烯酸酯和三唑类杀菌剂残留的风险水平,建立了高效液相色谱-串联质谱技术同时测定吡唑醚菌酯、苯醚甲环唑等13种杀菌剂的分析方法,并运用点评估法对儿童和成人的长期及短期膳食暴露风险进行评估。结果表明:13种杀菌剂在1~100 μg/L范围内线性关系良好,决定系数R2≥0.9994,定量限 (LOQ) 为5 μg/kg;在5种不同添加水平下,平均回收率为76%~122%,相对标准偏差(RSD)为0.7%~14%。残留检测结果显示:我国杧果主产区和消费城市1056份全果样品中检出率较高的杀菌剂有吡唑醚菌酯 (22.6%)、嘧菌酯 (18.5%) 和苯醚甲环唑 (15.5%)。运用杀菌剂50th和97.5th百分位点的残留值分别计算对长期摄入风险的贡献率和短期膳食摄入风险。结果表明:杧果中吡唑醚菌酯等13种杀菌剂对成人和儿童的长期摄入风险的贡献率分别为0.01%~0.5%和0.01%~0.3%,9种有急性参考剂量的杀菌剂残留在成人和儿童中短期暴露风险商分别为0.01%~7.2%和0.02%~28.9%,通过杧果摄入13种杀菌剂对人体产生的长期和短期膳食摄入风险较小。根据上述杀菌剂在我国杧果上的登记情况、GB 2763—2021《食品安全国家标准 食品中农药最大残留限量》以及膳食评估结果,建议加强对醚菌酯、丙环唑、腈菌唑、氟硅唑、腈苯唑、氟环唑和三唑醇7种杧果上未登记农药的监管,修订吡唑醚菌酯及制定肟菌酯等杀菌剂在杧果中最大残留限量(MRL)。

       

      Abstract: The spraying of fungicides on mangoes near harvest time to reduce occurrence of diseases could result in the pesticide residues in harvested products. To explore the potential health risk for subgroups in China, a residue analytical method was developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for simultaneous determination of 13 strobilurin and triazole fungicides in mango. Based on the residue data, the long-term and short-term dietary exposure risk assessment to adults and children in China was conducted by "point estimates" method. The linearity of 13 fungicides was excellent in the range of 1-100 μg/L, with the coefficients of determination (R2) above 0.9994. The limit of quantification (LOQ) was 5 μg/kg. The average recoveries were 76%-122% at 5 spiked levels, with the relative standard deviation (RSD) of 0.7%-14%. The residue results of the mango samples from the main producing areas and consumer cities in China indicated that the fungicides with high detection rates were pyraclostrobin (22.6%), azoxystrobin (18.5%) and difenoconazole (15.5%). The 50th and 97.5th percentile residue levels were used to calculate the contribution rate of the fungicides to the long-term and short-term dietary intake risks, respectively. The results demonstrated that the contribution rates of 13 fungicides in mango to the long-term dietary risk of adults and children were 0.01%-0.5% and 0.01%-0.3%, respectively. For the short-term assessment, the dietary exposure risks of the 9 fungicides that have acute reference dose (ARfD) were acceptable with risk quotients (RQs) of 0.02%-28.9% for children and 0.01%-7.2% for adults. From the risk assessment results, it could be concluded that long-term and short-term exposure of 13 fungicides in mangoes would not cause a health concern for general population. According to the registration status of the 13 pesticides, the MRLs in national standards of China (GB 2763—2021), and the results of dietary exposure assessment of the 13 fungicides, it is recommended to strengthen the supervision on application of 7 unregistered pesticides on mangoes, including kresoxim-methyl, propiconazole, myclobutanil, flusilazole, fenbuconazole, epoxiconazole and triadimenol, and to modify the MRL of pyraclostrobin and to set a MRL for picoxystrobin in mangoes.

       

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