Abstract: Triadimefon is an important pesticide for controlling powdery mildew of rubber tree. By clarifying the translocation and enrichment behaviors of triadimefon and its metabolite triadimenol on rubber trees, it is helpful to improve the target utilization rate of chemical control. In this study, the residue analytical method of triadimefon and triadimenol in the roots, stems and leaves of rubber tree was established for the first time based on the QuEChERS method and GC-MS/MS detection. The translocation and enrichment behaviors of triadimefon and its metabolite triadimenol in rubber plants were studied by means of root application under hydroponic culture conditions and foliar application under soil culture. The results showed that the plants of rubber tree could rapidly metabolize triadimefon to triadimenol, and both of the two chemicals could be translocated from the roots to the leaves through the stem, but their enrichment behaviors in each part of the plant were significantly different. The bioconcentration factors were in the order of root > stem > leaf, and the translocation factors of both stem and leaf were less than 1, indicating that triadimefon and its metabolite triadimenol were difficult to migrate upward in the plants of rubber tree and were easily enriched by roots. Triadimefon was also rapidly metabolized to triadimenol under foliar application conditions. However, the translocation ability between the leaves of different parts of the plant was poor for both of the two pesticides. This study provides important data for the optimization of the application scheme of triadimefon and other pesticides on rubber trees.