Abstract: With the implementation of pesticide reduction, plant-derived pesticides have received more and more attention because of their low toxicity and low residue. Limonene is a natural monocyclic terpene widely present in citrus essential oils, which has diverse biological activities, and has potential and application prospect in agricultural disease, pest and weed control. The research progress of limonene and its essential oil in the agricultural fields of insecticidal, acaricidal, phytocidal and antifungal activity in recent years, and the mechanism of its biological activities were briefly reviewed and summarized in this paper. Meanwhile, the registration of limonene formulations in China and the research progress and application of limonene nano-formulations against agricultural diseases and pests were introduced, and the future development trend and research direction of this field were prospected, which will provide the scientific basis for the application of limonene in pesticide reduction and agricultural disease, pest and weed control.