Abstract: L-Glufosinate is a new-type herbicide with high herbicidal activity, broad-spectrum and environmental friendliness. It has high cost efficiency and broader application prospects and can be synthesized by chemical synthesis and biocatalytic transformation. On the basis of the investigation of the synthesis methods of L-glufosinate in recent years, the research progress by different synthesis methods of L-glufosinate, such as chiral induction, chiral material, and asymmetric catalysis, were introduced. Finally, the future trends are discussed, which provides valuable information for the follow-up research of the L-glufosinate．