Abstract: An analytical method was developed for the simultaneous determination of carbosulfan and its metabolites (carbofuran and 3-hydroxycarbofuran) residues in tobacco leaf and soil by gas chromatography-mass spectrometry(GC-MS). Soil samples were extracted with acetone-petroleum ether V(acetone):V(petroleum ether)=1:4; Fresh tobacco samples and flue-cured tobacco leaf samples were extracted with acetone-acetonitrile V(acetone):V(acetonitrile)=1:9 and acetonitrile, respectively. The extracts were concentrated by rotary evaporation, and followed by cleanup on Florisil column. With fortified concentration range of 0.05-1 mg/kg, the average recoveries ranged from 74% to 99%, and with relative standard deviation (RSD, n=5) of 1.5%-9.2%. The pre-treatment step of the current method is relatively simple, and the accuracy, precision and sensitivity of the method meet with the technical requirements for the analysis and detection of pesticide residues. The method is suitable for analysis and detection of carbosulfan and its major metabolites in tobacco leaf and soil.