Abstract: In order to find some eco-friendly components from plants as mosquitocide, essential oil was extracted from leaves of Fokienia hodginsii was extracted, the insecticidal activity of the essential oil against Ⅳ larvae and pupae of Aedes albopictus and Culex pipiens quinquefasciatus were investigated by immersion method, and the fumigant activity of the essential oil to the adults of two mosquito species were evaluated by means of airtight cylinder. The compositions of the essential oil was analyzed by GC-MS. The results showed that the LC50 values of the essential oil were 53.21 and 144.34 μg/mL against Ⅳ instar larvae and pupae of A. albopictus, respectively, while the LC50 values were 55.85 and 147.68 μg/mL against Ⅳ instar larvae and pupae of C. pipiens quinquefasciatus, respectively. At the dosage of 4.36 μg/cm3, the KT50 values of essential oil to adults of A. albopictus and C. pipiens quinquefasciatus were 14.63 and 14.90 minutes, respectively, while the 24 hours corrected mortalities of adults of the two mosquito species were 90.00%. Sixteen chemical compounds were identified in F.hodginsii oil, the highest relative components of the essential oil wasα-pinene (29.25%), followed by bicyclo 2.2.1 hept-3-en-2-ol (10.88%) and caryophyllene oxide (9.64%). The results indicated that the essential oil extracted from F.hodginsii had strong insecticidal activity against A. albopictus and C. pipiens quinquefasciatus.