Abstract: In order to evaluate the toxicity effect of induced enzyme fermented solution of protease, chitinase, lipase and amylase from Beauveria bassiana, 3 types of solutions were prepared under laboratory conditions, i.e.,1×107/mLspore suspension (type Ⅰ), spore suspension + induced enzyme fermented solution (V:V=1: 1, type Ⅱ) and spore suspension + sterilized induced enzyme fermented solution (V:V=1:1, type Ⅲ), and microbial virulence against Monochamus alternatus larvae were tested by immersion method. The results showed that the LT50 values of induced protease, chitinase and lipase fermented solution(type Ⅱ)were significantly different from that of type Ⅲ solution (P50 values was not significantly different between the two types of solution. Moreover, there were significant difference between type Ⅲ and type Ⅰ fermented solution of 4 kinds of enzyme (PB. bassiana, and the inactivated 4 kinds of fermented solution also showed significant synergistic effect.