姬婧媛, 杨洁, 高小宁, 黄丽丽, 康振生. 植物内生枯草芽孢杆菌E1R-j脂肽类化合物的分离鉴定及抑菌作用[J]. 农药学学报, 2015, 17(2): 172-178. DOI: 10.3969/j.issn.1008-7303.2015.02.08
    引用本文: 姬婧媛, 杨洁, 高小宁, 黄丽丽, 康振生. 植物内生枯草芽孢杆菌E1R-j脂肽类化合物的分离鉴定及抑菌作用[J]. 农药学学报, 2015, 17(2): 172-178. DOI: 10.3969/j.issn.1008-7303.2015.02.08
    shu
    Ji Jingyuan, Yang Jie, Gao Xiaoning, Huang Lili, Kang Zhensheng. Isolation and identification of lipopeptides produced by endophytic bacteria Bacillus subtilis E1R-j and its anti-fungal mechanism studies[J]. Chinese Journal of Pesticide Science, 2015, 17(2): 172-178. DOI: 10.3969/j.issn.1008-7303.2015.02.08
    Citation: Ji Jingyuan, Yang Jie, Gao Xiaoning, Huang Lili, Kang Zhensheng. Isolation and identification of lipopeptides produced by endophytic bacteria Bacillus subtilis E1R-j and its anti-fungal mechanism studies[J]. Chinese Journal of Pesticide Science, 2015, 17(2): 172-178. DOI: 10.3969/j.issn.1008-7303.2015.02.08
    shu

    植物内生枯草芽孢杆菌E1R-j脂肽类化合物的分离鉴定及抑菌作用

    Isolation and identification of lipopeptides produced by endophytic bacteria Bacillus subtilis E1R-j and its anti-fungal mechanism studies

      摘要
    • 摘要: 植物内生枯草芽孢杆菌Bacillus subtilis菌株E1R-j分离自小麦根部。以小麦全蚀病菌作为靶标菌,采用酸沉淀、快速蛋白液相色谱(FPLC)和基质辅助激光解吸附飞行时间质谱(MALDI-TOF/TOF-MS)技术,对该菌发酵液中的抗真菌活性物质进行分离鉴定和抑菌活性研究。结果表明:菌株E1R-j产生的抗菌活性物质由P1、P2、P3和P4 4类组分组成,其中P3起主要抑菌作用。MALDI-TOF/TOF-MS分析结果显示,P3为fengycins。经试验发现,P3对温度稳定,121 ℃下放置30 min仍可保持抑菌活性。电子显微境观察发现,P3对小麦全蚀病菌菌丝生长有强烈的抑制作用,同时还可造成菌丝细胞畸形、细胞壁溃解、细胞质外渗等。

       

      Abstract: The entophytic Bacillus subtilis E1R-j was isolated from wheat roots. The objective of this study was to isolate and identify the lipopeptides produced by B. subtilis strain E1R-j using acid precipitation, FPLC and MALDI-TOF/TOF-MS techniques. Four antifungal fractions, P1, P2, P3 and P4, were separated from the lipopeptides, and the P3 fraction has been shown to bear the major antifungal activity. According to the mass spectra analysis, P3 consisted of fengycins. The further experiments demonstrated that P3 was stable to high temperature, and even remained antifungal activity at 121 ℃ for 30 min. Microscope observation revealed that the P3 strongly inhibited the growth of mycelial. Furthermore, it can also cause deformities of mycelial cells, disintegration of the cell wall, the leakage of cytoplasm and so on.

       

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