Abstract: A method of residue detection of dinotefuran in paddy water and soil by high performance liquid chromatographic (HPLC) was established and applied in the analysis of samples from Guangxi, Hunan and Anhui. Paddy water was filtrated by double filters and soil samples were extracted with acetonitrile-water, and the retention of dinotefuran in different chromatographic conditions was studied. The results showed that the excellent separation was obtained and the limit of detection (LOD) was 0.08 ng and the limit of quantification (LOQ) was 0.05 mg/kg. The range of average recovery was 74% to 83% and the relative standard deviations (RSD) was 1.6% to 5.9%. The dissipation dynamics of dinotefuran in paddy water accorded with the first-order kinetics equation, the half-life was 20.5 d(Guangxi), 4.5 d (Hunan) and 3.3 d (Anhui) . The original deposition was not detected in soil and the kinetic equation was not simulated.