Abstract: Bacteria BMJHZ-01, a strain capable of degrading difenoconazole was isolated from sewage sludges at a pesticide factory district in Jiangsu province. The morphology, physiological and biochemical identification for this strain was studied. 16S rDNA sequence homology analysis and phylogenetic tree alignment system indicated it as the Pseudomonas nitroreducens. Based on the study of the influence factors of degradation of difenoconazole, the most suitable degradation conditions for BMJHZ-01 was obtained as: Incubator speed 200 r/min and culture temperature of 30 ℃, initial pH value of 7.0. High performance liquid chromatography were used to detect pesticide concentration and its degradation rate was calculated. Difenoconazole with concentration of 180 mg/L could degraded more than 90% by the bacteria BMJHZ-01 within five days. BMJHZ-01 could grow and propagate in the highest difenoconazole concentration of 180 mg/L. The research results imply that this strain may be ultilized in future environmental management of difenoconazole pollution.