高效液相色谱法测定芦笋中多菌灵和吡虫啉残留

The Determination of Carbendazim and Imidacloprid Residues in Asparagus by High-Performance Liquid Chromatography

摘要: 建立了一种同时测定芦笋中多菌灵和吡虫啉农药残留的高效液相色谱法。以丙酮超声波提取,石油醚去除脂类杂质,再用二氯甲烷萃取分离,浓缩后测定。用甲醇-水(40∶60,体积比)为流动相,配备Discovery ODS-C18柱、紫外检测器的高效液相色谱(HPLC)仪对待测组份进行了分离和测定。实验证明,芦笋样品中多菌灵和吡虫啉添加回收率分别在81.0%~99.6%和79.3%~100.2%之间,相对标准偏差(RSD,n＝3)分别小于13.4%和8.0%,多菌灵和吡虫啉在样品中的最低检出浓度为0.05 mg/kg。

Abstract: An analytical method of carbendazim and imidacloprid residues in asparagus by high-performance liquid chromatography (HPLC) was developed. Samples were extracted with acetone by ultrasonic method, liquid-liquid extraction (LLE) cleanup with petroleum ether to remove some fatty impurities and then with dichloromethane to extract the samples further. The HPLC conditions were Discovery ODS-C18 column, methanol-water=40∶60 (V/V) as mobile phase, and UV detection at 280 nm. The fortified recoveries of carbendazim and imidaclorpid in asparagus samples were 81.0%~99.6% and 79.3%~100.2%, respectively. Relative standard deviation was less than 13.4% for carbendazim and 8.0% for imidaclorpid, and the limit of detection of two pesticides in asparagus samples was 0.05 mg/kg, respectively.