NYS-4菌株发酵液抑菌活性成分的分离及初步结构鉴定

张文娟; 王贞; 魏少鹏; 姬志勤; 吴文君

NYS-4菌株发酵液抑菌活性成分的分离及初步结构鉴定

Isolation and identification of antimicrobial components from broth of strain NYS-4

摘要

摘要: NYS-4菌株是从秦岭地区土壤中分离到的一株放线菌。采用管碟法及抑制菌丝生长速率法测定了NYS-4菌株发酵液对多种病原细菌和真菌的抑菌活性。结果表明:NYS-4菌株发酵液对白菜软腐病菌 Erwinia carotovora var.、猕猴桃溃疡病菌 Kiwi ulcer disease germs 和枯草芽孢杆菌 Bacillus subtilis 3种病原细菌具有较强的抑菌活性;对西瓜枯萎病菌 Fusarium oxysporum f.sp .Niveum 、烟草赤星病菌 Alternaria alternate 、油菜菌核病菌 Sclerotinia sclerotiorum 等8种病原真菌菌丝生长亦具有较强的抑制作用。采用离子交换树脂吸附、凝胶柱层析、反相高效液相色谱等方法,结合高效液相色谱-质谱联用(HPLC-ESI-MS/MS)技术,对NYS-4菌株发酵液的活性次生代谢物进行了分离和鉴定,从中分离鉴定出14个链丝菌素(streptothticin)类化合物,其中, N -乙酰化链丝菌素A、 N -乙酰化链丝菌素B、 N -乙酰化链丝菌素B酸和链丝菌素B酸经初步鉴定为新化合物。

Abstract: An actinomycete strain NYS-4 was isolated from a soil sample collected from the Qinling mountain of Shaanxi province of China.Antimicrobial activities of the fermentation broth of strain NYS-4 were investigated by methods of cylinder plate and inhibition of mycelial growth rate.The result of bioassay indicated that the broth of NYS-4 exhibited strong antimicrobial activities against several pathogens,such as Erwinia carotovora var., Kiwi ulcer disease germs,Bacillus subtilis,Fusarium oxysporum f.sp. Niveum,Alternaria alternate and Sclerotinia sclerotiorum .Purified by anion-exchange resin,gel column chromatography,ion-pair reversed-phase and HPLC-ESI-MS/MS,fourteen streptothticins including four new compounds, N -acetyl streptothticin A, N -acetyl streptothticin B, N -acetyl streptothticin B acid and streptothticin B acid,were isolated and identified from the metabolites of strain NYS-4 by bioassay-guided fractionation.

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