Abstract: In order to explore the application prospects of Daphniphyllum calycinum in controlling agricultural disease,ten compounds were isolated from acetic ether-soluble fraction of D.calycinum by column chromatography and recrystallization,and three plant pathogens (Rhizoctonia solani,Sclerotium rolfsii,Fusarium oxysporum f.sp.cubense) were used to screen antifungal activity of the compounds.The structure of ten compounds were identified as p-methoxybenzoic acid (1),p-hydroxybenzaldehyde (2),5,7-dihydroxychromone (3),p-hydroxybenzoic acid (4),trans-p-hydroxylcinnamic acid (5),5,7,4'-trihydroxy-3'-methoxyflavone (6),kaempferol (7), β-daucosterol (8),luteolin (9) and 3,4-dihydroxybenzoic acid (10) by NMR.The antifungal activities of nine compounds against the three plant pathogens were tested.At the concentration of 100 mg/L,compound 3,6,7 and 10 exhibited antifungal activity against R.solani,with inhibitory rates of 58.1%,74.3%,85.0% and 79.5%,respectively;compound 6 and 7 showed potential antifungal activity against S.rolfsii,with inhibitory rates of 66.2% and 72.5%,respectively;compound 6 had weak antifungal activity against F.oxysporum,with inhibitory rate of 54.5%.Compound 1-7,9-10 were obtained from the fruit of D.calycinum for the first time.