Abstract: Triazole fungicides are widely used fungicides in the world. The vast majority of triazole fungicides are chiral pesticides with one or two chiral centers and two or four enantiomers. Based on the research results of triazole fungicides in recent years, this article summarized the chromatographic methods such as high performance liquid chromatography, supercritical fluid chromatography, capillary electrophoresis, gas chromatography, and convergence chromatography for the chiral analysis of 11 triazole fungicides with two chiral centers including epoxiconazole, paclobutrazol, triadimenol, bitertanol, propiconazole, difenoconazole, metconazole, diclobutrazol, cyproconazole, bromuconazole, and etaconazole. The research progress in the separation and determination of triazoles with different chiral centers can provide references for the separation, analysis, and in-depth study of triazole compounds with multiple chiral centers.