Abstract: Bio-sourced herbicides are environmentally friendly herbicides, which are one of the development direction of herbicides in the future. From the perspective of the application of bio-sourced herbicides, the research progress on bio-sourced herbicidal active substances in the field of weed control over the years were summarized, and the present status of development and application status of bio-sourced herbicidal active substances and their derivatives were systematically summarized. Plant-derived herbicidal active substances include the excretions, extracts and chemically modified derivatives of Pinaceae, Myrtaceae, Rutaceae, Asteraceae plants and so on. Microbial-derived herbicidal active substances include fungi, bacteria, actinomycetes, viruses and their secondary metabolites. This review could provide some theoretical basis for the development and application of bio-sourced herbicides.