李向楠, 吴振兴, 陈坚剑, 郭国锦, 梅高甫, 王剑, 吕桂华. 耐性和敏感两种类型鲜食玉米苗期施用硝磺草酮后的转录组分析[J]. 农药学学报, 2021, 23(5): 893-904. DOI: 10.16801/j.issn.1008-7303.2021.0133
    引用本文: 李向楠, 吴振兴, 陈坚剑, 郭国锦, 梅高甫, 王剑, 吕桂华. 耐性和敏感两种类型鲜食玉米苗期施用硝磺草酮后的转录组分析[J]. 农药学学报, 2021, 23(5): 893-904. DOI: 10.16801/j.issn.1008-7303.2021.0133
    LI Xiangnan, WU Zhenxing, CHEN Jianjian, GUO Guojin, MEI Gaofu, WANG Jian, LÜ Guihua. RNA-Seq transcriptome analysis of fresh-eating maizes sensitive and tolerant to mesotrione in the seedling stage[J]. Chinese Journal of Pesticide Science, 2021, 23(5): 893-904. DOI: 10.16801/j.issn.1008-7303.2021.0133
    Citation: LI Xiangnan, WU Zhenxing, CHEN Jianjian, GUO Guojin, MEI Gaofu, WANG Jian, LÜ Guihua. RNA-Seq transcriptome analysis of fresh-eating maizes sensitive and tolerant to mesotrione in the seedling stage[J]. Chinese Journal of Pesticide Science, 2021, 23(5): 893-904. DOI: 10.16801/j.issn.1008-7303.2021.0133

    耐性和敏感两种类型鲜食玉米苗期施用硝磺草酮后的转录组分析

    RNA-Seq transcriptome analysis of fresh-eating maizes sensitive and tolerant to mesotrione in the seedling stage

    • 摘要: 苗期施用硝磺草酮易对鲜食玉米品种和自交系造成药害。鲜食玉米对硝磺草酮的不同响应差异可能归因于除草剂代谢效率的不同。本研究从氧化损伤和光合抑制角度阐述了不同耐药性鲜食玉米对硝磺草酮的响应差异。利用RNA-Seq技术对耐性和敏感玉米在硝磺草酮处理和未处理下的基因表达情况进行了分析,以期从生理和分子水平揭示鲜食玉米对硝磺草酮的耐性机制。结果表明:硝磺草酮处理导致敏感玉米自交系叶片中积累更多的活性氧,光合作用受到严重抑制,最终植株干枯死亡;而耐性玉米自交系在硝磺草酮处理后植株生长正常。本研究中,共鉴定到7985个差异表达基因,敏感玉米中上调表达基因4020个、下调表达基因3450个,而耐性玉米中上调表达基因526个、下调表达基因495个;差异表达基因的基因功能注释 (GO) 富集分析表明:差异表达基因数量较多的主要集中在分子功能、细胞组分、生物学过程中;京都基因与基因组百科全书 (KEGG) 分析显示:差异表达基因显著富集在光合作用、碳水化合物、氨基酸和脂类代谢等相关通路;筛选到Zm00001d042099Zm00001d044157Zm00001d007462Zm00001d002436Zm00001d021804Zm00001d042541Zm00001d040764Zm00001d032828Zm00001d020544共9个可能参与除草剂代谢的潜在候选基因。

       

      Abstract: Postemergence applications of mesotrione can cause great damage to maize inbred lines and hybrids. Variation among different responses to mesotrione may be attributed to differential rates of herbicide metabolism. In this study, field and pot experiments were conducted using two fresh-eating maize inbred lines with different response to mesotrione. After mesotrione treatment, the accumulation of ROS in susceptible maize inbred line were significantly increased. The photosynthesis was severely inhibited, and the susceptible plant eventually died. We employed RNA-Seq analysis to compare transcriptome responses between mesotrione-treated and untreated in both tolerant and susceptible maize inbred lines. A total of 7985 differentiation expressed genes (DEGs) were identified. There were 4020 up-regulated genes and 3450 down- regulated genes in susceptible maize, and only 526 up-regulated genes and 495 down-regulated genes in tolerant maize. Gene oncology (GO) enrichment analysis showed that the majority of differentiation expressed genes were concentrated in molecular function, cellular components and biological processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the differentiation expressed genes were mainly involved in photosynthesis, carbohydrate, amino acid and lipid metabolism pathways. Based on gene expression and function annotation, 9 genes were selected as the candidates most likely involved in herbicide metabolism, and quantitative real time RT-PCR validated the RNA-Seq results. The candidate genes were Zm00001d042099, Zm00001d044157, Zm00001d007462, Zm00001d002436, Zm00001d021804, Zm00001d042541, Zm00001d040764, Zm00001d032828 and Zm00001d020544.

       

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