致病杆菌属细菌代谢物抑菌活性研究进展

韩云飞; 他永全; 王勇; 冯俊涛; 王永红

doi:10.16801/j.issn.1008-7303.2021.0192

致病杆菌属细菌代谢物抑菌活性研究进展

doi: 10.16801/j.issn.1008-7303.2021.0192

韩云飞^1,,
他永全¹,
王勇^{1, 2},
冯俊涛^{1, 2},
王永红^{1, 2, ,}

1.
西北农林科技大学植物保护学院，陕西杨凌 712100
2.
陕西省生物农药工程技术研究中心，陕西杨凌 712100

基金项目: 国家自然科学基金(32072474)；国家重点研发计划(2017YFD0201203).

详细信息

作者简介:
韩云飞，hanyunfei@nwafu.edu.cn

通讯作者:
王永红，yhwang@nwafu.edu.cn.

中图分类号: S476.1；S482.292
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- 被引次数: 0
出版历程
- 收稿日期: 2021-09-27
- 录用日期: 2021-11-04
- 网络出版日期: 2021-12-24
- 刊出日期: 2022-04-01

Advances in antimicrobial substances from genus Xenorhabdus

HAN Yunfei^1
,,
TA Yongquan¹,
WANG Yong^{1, 2},
FENG Juntao^{1, 2},
WANG Yonghong^{1, 2
, ,}

1.
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
2.
Shaanxi Research Center of Biopesticide Engineering & Technology, Northwest A&F University, Yangling 712100, Shaanxi Province, China

Funds: the National Natural Science Foundation of China (32072474), the National Key Research and Development Program of China (2017YFD0201203).

摘要

摘要: 致病杆菌属 (Xenorhabdus) 细菌是一类存在于昆虫病原线虫肠道内的共生菌，能够产生多种具有不同生物活性的次级代谢产物，其中一些化合物具有开发成为新农药的潜力。本文综述了致病杆菌属细菌中可产生抑菌活性物质的代表性菌株，较为全面地总结了近几十年来在致病杆菌属细菌代谢物中发现的抑菌活性化合物；对致病杆菌属细菌产生的部分抑菌活性化合物的抑菌作用机理进行了讨论，认为影响蛋白质合成是某些化合物发挥抑菌作用的重要途径。基于抑菌活性化合物的生物合成途径及相关调控因子，总结了从致病杆菌属细菌代谢物中发掘新化合物和提高特定活性化合物产量的方法。针对现阶段致病杆菌属细菌及其次级代谢产物研究应用中存在的问题提出了相应的对策，并简要概述了未来致病杆菌属细菌的研究发展方向。本文可为如何合理有效利用致病杆菌属细菌活性代谢物提供参考，对于促进致病杆菌属细菌及其次级代谢产物的应用具有重要意义。
- 致病杆菌 /
- 昆虫病原线虫共生菌 /
- 代谢物 /
- 抑菌活性 /
- Xenocoumacin
Abstract: Xenorhabdus is a kind of symbiotic bacteria living in the intestinal tract of entomopathogenic nematodes and produces a variety of secondary metabolites with different biological activities, some of which have the potential to develop new pesticides. Antimicrobial compounds found in the secondary metabolites from Xenorhabdus genus in recent decades and representative strains that produced antimicrobial secondary metabolites were summarized in this paper. The mechanisms of some antimicrobial compounds produced by Xenorhabdus were discussed and the inhibition of protein synthesis was an important way for some compounds against microorganisms. The methods of exploring new compounds and improving the yield of specific antimicrobial compounds from secondary metabolites of genus Xenorhabdus were summarized based on their biosynthesis pathway and related regulatory factors. The research prospects of Xenorhabdus were generalized and some countermeasures were put forward in this article in view of the current problems in the research and application of Xenorhabdus and its secondary metabolites. This paper provides a reference for the reasonable and effective application of antimicrobial secondary metabolites of Xenorhabdus, which is of great significance in promoting the utilization of Xenorhabdus and its secondary metabolites.
- Xenorhabdus /
- symbiotic bacteria of entomopathogenic nematodes /
- metabolite /
- antimicrobial activity /
- Xenocoumacin

HTML全文

图 1 嗜线虫致病杆菌YL001初生型菌株在NBTA培养基上的形态特征

Figure 1. Characteristics of primary phenotypic X. nematophila YL001 on NBTA medium

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图 2 致病杆菌属细菌产生的抑菌活性化合物 (1~25)

Figure 2. Antimicrobial compounds (1-25) produced by genus Xenorhabdus

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图 3 致病杆菌属细菌产生的多肽类抑菌活性化合物 (26~54)

Figure 3. Polypeptide antimicrobial compounds (26-54) produced by genus Xenorhabdus

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图 4 致病杆菌属细菌产生的酰胺类及其他抑菌活性化合物 (55~64)

Figure 4. Amides and others antimicrobial compounds (55-64) produced by genus Xenorhabdus

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表 1 致病杆菌属细菌发酵液的抑菌活性

Table 1. Antimicrobial activities of the fermentation liquid of genus Xenorhabdus

菌株　　　　　 Strains	菌株发现地　　 Places of Xenorhabdus discovered	抑菌谱　 Bacteriostatic spectrum	文献 References
伯氏致病杆菌 YL002 Xenorhabdus bovienii YL002	中国杨凌 Yangling, China	真菌、细菌、卵菌 Fungi, bacteria, oomycetes	[11]
Xenorhabdus budapestensis C72	中国北京 Beijing, China	真菌 Fungi	[27]
Xenorhabdus cabanillasii	土耳其 Turkey	真菌 Fungi	[28]
Xenorhabdus doucetiae IBSC n15	巴西 Brazil	真菌 Fungi	[29]
Xenorhabdus khoisanae J194	南非 South Africa	细菌 Bacteria	[30]
Xenorhabdus khoisanae SB10	南非 South Africa	真菌、细菌 Fungi, bacteria	[31]
嗜线虫致病杆菌 CB6 Xenorhabdus nematophila CB6	中国北京 Beijing, China	真菌、细菌、卵菌 Fungi, bacteria, oomycetes	[14, 32]
嗜线虫致病杆菌 YL001 Xenorhabdus nematophila YL001	中国杨凌 Yangling, China	真菌、细菌、卵菌 Fungi, bacteria, oomycetes	[11, 17]
Xenorhabdus romanii CER129	巴西 Brazil	真菌 Fungi	[29]
Xenorhabdus sp. strain Q1	澳大利亚 Australia	真菌、细菌 Fungi, bacteria	[4]
Xenorhabdus szentirmaii DSM 16338	阿根廷 Argentina	真菌 Fungi	[33]

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