Abstract: Triflumezopyrim is a new type of mesoionic insecticide, which is mainly used for the control of piercing-sucking pests such as rice planthoppers and leafhoppers. It has good uptake and translocation ability. To clarify the uptake and translocation of triflumezopyrim in different paddy rice and provide a scientific basis for the reduction of its application and increase of its efficiency, Yangdao 6 and Nanjing 46 were used as the test paddy rice and the differences in the uptake and translocation of triflumezopyrim on them were explored by hydroponics and leaf soaking methods. The hydroponics treatment of paddy rice seedlings showed that the uptake capacity of triflumezopyrim by the roots of Yangdao 6 was stronger than that of Nanjing 46, but the ability of upward translocation was significantly lower than that of Nanjing 46. The results of the leaf soaking method showed that triflumezopyrim was not detected in the roots of two kinds of paddy rice, and the content of triflumezopyrim in leaves and stems of Yangdao 6 was significantly lower than that of Nanjing 46, but there was no significant difference in downward translocation ability. The results showed that triflumezopyrim could be absorbed by the roots and leaves of two kinds of paddy rice, and had an excellent ability for translocation to the top, but weak downward translocation ability. The top and down translocation capacity of triflumezopyrim in Nanjing 46 was better than that in Yangdao 6.