Abstract: Trichoderma asperellum TCS007 strain was isolated from marine extreme environments and was domesticated and mutagenic stabilized. It is known that this strain and its metabolites have broad-spectrum inhibitory activity against plant pathogenic fungi and have the capabilities of inducing plant growth and resistance, exhibiting a great development prospect. In order to explore the optimal fermentation conditions for the spore production of TCS007 strain in solid-state fermentation, and define the antifungal activity of the metabolites of TCS007 strain, the formulation of TCS007 strain solid fermentation medium and fermentation culture conditions were optimized by a one-way test combined with the response surface method, and then the antifungal activities of the solid-state fermentation filtrate and crude extract against six plant pathogenic fungi were determined by the mycelial growth rate method. The experimental results showed that the three main factors affecting the spores yield of solid fermentation of TCS007 strain were incubation time, water addition and light cycle time. The optimized fermentation substrate was wheat bran + soybean meal, with a mass ratio of 5 : 1, supplied with 1% MnSO₄ and 1% MgSO₄; the optimized fermentation conditions were 15 d of incubation time, 39.7 mL of water addition, 21 h of light cycle time, 28 ℃ of culture temperature, 2 mL of inoculum (1 × 10⁷ spores/mL), and the initial pH value 5.0. Under these fermentation conditions, the spore yield of TCS007 strain was 7.14 × 10⁹ spores/g, which was fitted to the spore yield predicted by the response surface methodology (6.85 × 10⁹ spores/g), and a 6-fold increase in spore yield over the initial fermentation condition. The antifungal assays further showed that both TCS007 strain solid-state fermentation filtrate (10-fold dilution) and crude extract (10 mg/L) showed good inhibitory effect against Sclerotinia sclerotiorum, with inhibition rates of 92.4% and 95.8%, respectively. These findings will provide the theoretical basis for the study of the fermentation process of the subsequent products and the development of TCS007 strain fungicide.