Abstract: Bacillus velezensis TCS001 is a new type of marine biocontrol bacterium, which has broad-spectrum antifungal activity promotes plant growth, and has great potential for development in agricultural applications. In this study, the additives and proportions of wetting and dispersing additives, thickeners, and preservatives with high compatibility with TCS001 were screened using the toxic medium method, growth determination method and suspension performance evaluation method, and the formula of TCS001 suspension agent (TCS001-SC) was obtained. The screening results showed that the TCS001-SC formula was: 40% TCS001 fermentation broth (≥10¹⁰ CFU/mL), 3% wetting and dispersing agent SXC, 3% wetting and dispersing agent UNA, 0.15% thickener xanthan gum, 0.2% preservative Kathone, 0.2% preservative sodium benzoate. When the water was supplemented to 100% (mass concentration), the number of viable bacteria of TCS001-SC developed was 4.0 × 10⁹ CFU/mL, with a miscellaneous bacteria rate of 4.8%. The pH value was 6.68, and the suspension rate was 97.8%. The pass rate through the 75 μm test sieve was 99.9%, and the mass fraction of residue after pouring was 1.6%, and after washing was 0.41%. The long-lasting foaming property (after 1 min) was 16 mL. All performances are in line with industry standards (NY/T 2989—2016). At the same time, the storage stability of TCS001-SC was measured at different temperatures, there was no significant difference in the number of viable bacteria when stored at (0 ± 0.2) ℃ for 7 days and (4 ± 0.2) ℃ for 360 days compared to before storage. The survival rate of bacterial cells was above 80% when stored at (28 ± 0.2) ℃ for 360 days and (35 ± 0.2) ℃ for 84 days, indicating good storage stability. On this basis, the field efficacy test was carried out with strawberry anthracnose as the target, and the test results showed that TCS001-SC 1500~3000 mL/hm² spray treatment could significantly reduce the occurrence of strawberry anthracnose, and the control effect was between 59.37%~79.52%. Among them, the control effect of 3000 mL/hm² spray treatment on strawberry anthracnose reached 79.52%, which provided a theoretical basis for the registration of TCS001-SC as a microbial fungicide for the prevention and control of strawberry anthracnose.