Abstract: Bacillus velezensis TCS001 is a new type of marine biocontrol bacterium, which has broad-spectrum antifungal activity and promotes plant growth, and has great potential for development in agricultural applications. In this study, the additives and proportions of wetting and dispersing additives, thickeners, and preservatives with high compatibility with TCS001 were screened using the toxic medium method, growth determination method and suspension performance evaluation method, and the formula of TCS001 suspension agent (TCS001 SC) was obtained. The screening results showed that the TCS001 SC formula was: 40% TCS001 fermentation broth (≥10¹⁰ CFU/mL), 3% wetting and dispersing additives SXC, 3% wetting and dispersing additives UNA, 0.15% thickener xanthan gum, 0.2% preservative Kathone, 0.2% preservative sodium benzoate. When the water was supplemented to 100% (mass fraction), the number of viable bacteria of TCS001 SC developed was 4.0 × 10⁹ CFU/mL, with a miscellaneous bacteria rate of 4.8%. For TCS001 SC, the pH value was 6.68, and the suspension rate was 97.8%. The pass rate through the 75 μm test sieve was 99.9%, and the mass fraction of residue after pouring was 1.6%, and after washing was 0.41%. And the long-lasting foaming property (after 1 min) was 16 mL, all performances are in line with industry standards (NY/T 2989—2016). Further more, the storage stability of TCS001 SC was measured at different temperatures, there was no significant difference in the number of viable bacteria when stored at (0 ± 0.2) ℃ for 7 days and (4 ± 0.2) ℃ for 360 days compared to before storage respectively, and the survival rate of bacterial cells was above 80% when stored at (28 ± 0.2) ℃ for 360 days and (35 ± 0.2) ℃ for 84 days, respectively indicating good storage stability. On this basis, the field test was carried out with strawberry anthracnose as the target, and the results showed that TCS001 SC 1500-3000 mL/hm² could significantly reduce the occurrence of strawberry anthracnose, and the control efficacy was between 59.37%-79.52%. Among them, the control efficacy of 3000 mL/hm² spraied 3 times at 7 d intervals on strawberry anthracnose reached 79.52%, which provided a theoretical basis for the registration of TCS001 SC as a microbial fungicide for the prevention and control of strawberry anthracnose.