河北省多主棒孢对3种常用杀菌剂的抗性及替代药剂对黄瓜棒孢叶斑病的防治效果

    Resistance of Corynespora cassiicola of Hebei Province to three common fungicides and the control efficacy of alternative fungicides on cucumber target leaf spot

    • 摘要: 为明确河北省黄瓜主产区多主棒孢对3种常用杀菌剂 (啶酰菌胺、肟菌酯和腐霉利) 的抗性水平并筛选有效防治药剂,采用菌丝生长速率法检测了2020—2021年河北省唐山、邯郸地区多主棒孢对啶酰菌胺、肟菌酯和腐霉利的敏感性,利用PCR技术分析了CcSdhCcCytb抗性突变基因型,采用盆栽试验评价了10种常用杀菌剂对该地区主流抗性突变体SdhB-I280V&Cytb-G143A的防治效果,并进行高效替代药剂交替使用的防效试验。结果表明:河北省唐山、邯郸黄瓜主产区的多主棒孢对啶酰菌胺、肟菌酯和腐霉利均产生了不同程度的抗性。其中,啶酰菌胺对280株多主棒孢的平均EC50值为8.12 μg/mL,抗性频率为27.43%;肟菌酯对144株多主棒孢的平均EC50值为62.6 μg/mL,对162株多主棒孢 (含EC50值大于200 μg/mL的18株菌) 的抗性频率为99.49%;腐霉利对163株多主棒孢的平均EC50值为6.95 μg/mL,抗性频率为52.76%。共检测出11种CcSdh抗性突变类型,突变频率达到56.86%。其中6种为单突变,4种为双突变,1种为三突变。供试多主棒孢CcCytb仅携带G143A突变,发生频率为100%。盆栽药效试验中,两种混配药剂75%百菌清可湿性粉剂 (WP) + 50%腐霉利WP和50%异菌脲WP + 75%吡醚 • 丙森锌WP对黄瓜棒孢叶斑病的防效最高,分别为71.52%和70.52%;其次是复配药剂75%吡醚 • 丙森锌WP,防效为57.90%;两种混配药剂75%百菌清WP + 50%腐霉利WP和50%异菌脲WP + 75%吡醚 • 丙森锌WP交替使用对黄瓜棒孢叶斑病的盆栽防效和田间防效分别为71.53%和94.64%。表明不同作用机制的杀菌剂混配并交替使用对黄瓜棒孢叶斑病的防治具有广阔应用前景。

       

      Abstract: In order to clarify resistance of Corynespora cassiicola to commonly used fungicides such as boscalid, trifloxystrobin and procymidoneoxime and screen effective fungicides, the sensitivity of C. cassiicola in Tangshan and Handan area of Hebei Province from 2020 to 2021 was detected by mycelium growth rate method, CcSdh and CcCytb resistant mutant genotypes were analyzed by PCR technique, the control efficacy of 10 commonly used fungicides against the local main resistant mutant SdhB-I280V&Cytb-G143A was evaluated by pot experiment, and the efficacy of the alternate use of highly effective alternative fungicides was carried out. According to the test results, the C. cassiicola in the main producing areas of cucumber in Tangshan and Handan of Hebei Province developed different degrees of resistance to boscalid, trifloxystrobin and procymidoneoxime. The average EC50 value of boscalid against the 280 strains of C. cassiicola was 8.12 μg/mL, with the resistance frequency was 27.43%. The average EC50 value of trifloxystrobin against the 144 strains of C. cassiicola was 62.6 μg/mL, with the resistance frequency against the 162 strains (including the 18 strains with EC50 value greater than 200 μg/mL) was 99.49%. The average EC50 of procymidoneoxime against the 163 strains of C. cassiicola was 6.95 μg/mL, with the resistance frequency was 52.76%. Eleven kinds of CcSdh resistance mutant types were detected in total, and the mutation frequency reached 56.86%. Six of them were single mutations, four of them were double mutations, and one of them was triple mutation. CcCytb carried only G143A mutation, and the frequency was 100%. In the pot experiment, the two mixed fungicides 75% chlorothalonil WP + 50% procymidone WP and 50% iprodione WP + 75% (pyraclostrobin + propineb) WP had the highest control efficacy on cucumber target leaf spot, with the control efficacy of 71.52% and 70.52%, respectively. The second was the compound fungicide 75% (pyraclostrobin + propineb) WP, and the control efficacy was 57.90%. 75% chlorothalonil WP + 50% procymidone WP and 50% iprodione WP + 75% (pyraclostrobin + propineb) WP were used alternately to control cucumber target leaf spot with 71.53% effect and 94.64% efficacy in pot experiment and field experiment, respectively. This showed that the mixing and alternate use of fungicides with different action mechanisms have broad application prospects for the control of cucumber target leaf spot.

       

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