绿茶种植-加工-冲泡过程中溴虫氟苯双酰胺的残留特征

    Residue behavior of broflanilide in green tea during planting, processing and brewing

    • 摘要: 本研究评价了溴虫氟苯双酰胺在绿茶种植-加工-冲泡过程中的残留转移规律。开展了270 mL/hm2和405 mL/hm2两个施药剂量的100 g/L溴虫氟苯双酰胺悬浮剂田间规范残留试验,于施药后不同时间采集茶鲜叶,加工制成绿茶,用于茶叶加工、冲泡试验。样品经乙腈提取、固相萃取柱净化,采用超高效液相色谱-串联质谱 (UPLC-MS/MS) 测定,基质匹配标准溶液定量。结果表明:溴虫氟苯双酰胺在茶叶中的消解动态符合一级动力学方程,半衰期仅1.9 d;药后7 d,溴虫氟苯双酰胺在茶鲜叶中的消解率达90%。溴虫氟苯双酰胺在从茶鲜叶到绿茶成茶过程中具有加工浓缩效应,总加工因子在4.09~4.94之间;绿茶加工过程中溴虫氟苯双酰胺的干重残留量没有显著变化,其消解率低于6%。溴虫氟苯双酰胺在绿茶冲泡过程中的浸出率<15%,其浸出率高低与茶叶整碎程度相关,整茶不同冲泡次数间的浸出率基本一致,而碎茶浸出率随冲泡次数的增加而减小;整茶中的总浸出率 (5%) 显著低于碎茶中的 (15%)。

       

      Abstract: This study evaluated the residual transfer behavior of broflanilide during tea planting, green tea processing and brewing. The supervised field trials of 100 g/L broflanilide suspension concentrate on tea were carried out at dosages of 270 mL/hm2 and 405 mL/hm2, and fresh leaves with different preharvest intervals were sampled and manufactured to green tea for tea processing and brewing test. The residues of broflanilide in tea samples were determined by extraction with acetonitrile, cleaning-up with solid phase extraction (SPE) column, and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. The matrix-matched calibration curves were used for the quantification. The results showed that the dissipation dynamics of broflanilide in fresh tea leaves were in accordance with the first-order kinetic equation, with the half-life of 1.9 d. On the 7th day after the application, 90% of the broflanilide was dissipated in the fresh tea leaves. The residue of broflanilide was concentrated during tea processing due to the loss of water, with the processing factor ranging from 4.09 to 4.94. Broflanilide was hardly degraded during green tea processing steps, with the loss rates lower than 6%. During the brewing of green tea, less than 15% of the broflanilide was brewed from the green tea to infusion. The leaching rate of broflanilide was correlated to the crushing degree of dry tea. The leaching rate of whole tea was basically the same among different brewing times, while the leaching rate of crushed tea decreased with the increase of brewing times. The total leaching rate in intact tea (5%) was lower than that in crushed tea (15%).

       

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