Abstract: Alkaloids are a class of natural products that are widely distributed in nature that have received considerable attention due to their unique biological activities. In recent years, research into the agroactivity of alkaloids has gradually become an important area of study in plant protection and sustainable agricultural development. In this paper, the research progress of agricultural activities of alkaloids such as berberine, cryptolepine, tylophorine and so on, which are applied to antimicrobial, antiviral, insecticidal and weeding, is systematically reviewed. To provide new ideas and methods for alkaloid-based agroactivity research, this paper summarizes the agroactivity and mechanism studies of alkaloids, and discusses the future research direction for alkaloid pesticides.