Abstract: In this study, the secondary metabolites of Streptomyces daghestanicus SL101 were isolated and purified using techniques including silica gel column chromatography, gel column chromatography and high performance liquid chromatography, and compounds 1-4 were obtained. Their structures were elucidated by a comprehensive analysis of high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance hydrogen spectrometer (¹H NMR) and carbon spectrometer (¹³C NMR) data, and comparison with literature. Compounds 1 and 2 were identified as sesquiterpenoids with a deep aspartic skeleton, and compounds 3 and 4 were classified as butylenolides. Among them, compounds 2 and 4 are new compounds, designated as (5R)-albaflavenol C and MKN-003C(B), respectively. The inhibitory effect of the four compounds on Pyricularia oryzae was determined using the mycelial growth rate method, and the results showed that compound 2 has better mycelial inhibition activity against P. oryzae, with the EC₅₀ value of 10.60 μg/mL. Furthermore, the isolated leaf method was employed to examine the control efficacy of compound 2 against rice blast, which showed a disease control efficacy of (74.1 ± 1.7)% , indicating its potential for further investigation.