Abstract: A high performance liquid chromatography (HPLC-UVD) method was established for the analysis of cycloxaprid residues in citrus and soil. Citrus samples were extracted with dichloromethanedistillate (2:1, V/V) and cleaned up by Florisil adsorption column and detected by HPLC-UVD. And soil samples was detected directly after the extraction. The external standard method was adopted for the quantification. This analytical method was used to determine the cycloxaprid residue in field samples. The results showed that there was a good linear relationship between the target substance concentration and the peak area in the range of 0.05-5 mg/L, The linear equation was y = 10 243x + 233.0 (R2 = 0.999 0), and the minimum detectable concentration in citrus and soil was both 0.1 mg/kg. The average recoveries in citrus and soil were from 76% to 93% at the fortified level of 0.1 mg/kg to 1 mg/kg, and the relative standard deviation (RSD, n = 5) was between 1.6% to 5.8%. The detection method was rapid, sensitive and stable. Thus it could be applied to determine cycloxaprid residues in soil and citrus.