Abstract: The transformation of acetamiprid during Agaricus bisporus industrial production was investigated by testing its residues in cover-soil, compost and fruits using ultra performance liquid chromatography (UPLC-PDA). The samples were extracted with acetonitrile and purified by dispersive solid phase. Acetamiprid residue was then determined by UPLC-PDA. At 0.02-3 mg/kg spiked levels, the average recoveries for cover-soil, compost and fruits were from 81% to 97% (RSDs ≤9.6%). In different application levels, the dissipation of acetamprid in soil and compost was found to conform to the first order kinetics equation and the average half-life was 34 d in cover-soil and 23 d in compost. The acetamprid applied in cover-soil moved into compost. And residues in the compost increased in the first 21-28 d and then started to decrease. At the application rate of 10-250 mg a.i./kg in soil or compost, acetamprid residues in fresh mushroom fruits were 0.03-2.6 mg/kg. And there were significant residue differences among the first, second and third harvesting time stages. At the same pesticide concentration, the acetamiprid residue level in fruit bodies is significantly higher when the pesticide was applied in the cover-soil compared to that when applied in the compost.