双氧木脂素E对东方粘虫幼虫体壁肌超微结构及Na+-K+-ATPase、Ca2+-ATPase的影响

肖新敏; 胡兆农; 姬志勤; 魏少鹏; 吕敏; 师宝君; 吴文君

doi:10.16801/j.issn.1008-7303.2017.0067

双氧木脂素E对东方粘虫幼虫体壁肌超微结构及Na+-K+-ATPase、Ca2+-ATPase的影响

Ultrastructure and Na+-K+-ATPase, Ca2+-ATPase activities on parietal muscle of Mythimna separata larvae treated by haedoxane E

摘要

摘要: 双氧木脂素E（haedoxane E）是从杀虫植物透骨草Phryma leptostachya L.中分离出的一种对多种农林害虫和卫生害虫具有杀虫活性的化合物。本研究以东方粘虫Mythimna separata为供试昆虫，对双氧木脂素E对其幼虫杀虫作用部位进行了初步研究。电镜观察表明，双氧木脂素E对东方粘虫幼虫体壁肌具有致毒作用，能使肌细胞发生明显病变，细胞核皱缩变形，染色质浓缩，线粒体肿胀、大面积空泡化，肌质网扩张，肌原纤维排列紊乱，胞质内出现大量多泡体。酶活力测定结果也显示，双氧木脂素E能不同程度抑制Na+-K+-ATPase和Ca2+-ATPase活力。因此推测双氧木脂素E是一种作用于东方粘虫肌肉组织的肌肉毒剂。

Abstract: Haedoxane E, isolated from Phryma leptostachya L. exhibited significant insecticidal activity against some insect species. In this paper, the action mode of haedoxane E to Mythimna separata larvae was studied. Transmission electron microscopy revealed that haedoxane E could induce evident pathological changes in muscle cells, including nucleus pyknosis and deformation, chromatin condensation, serious disruption of mitochondria, sarcoplasmic reticulum dilation, disordered arrangement of myofibril, and the appearance of numerous multivesicular bodies. At the same time, haedoxane E inhibited the activities of Na+-K+-ATPase and Ca2+-ATPase. Therefore, haedoxane E is possibly a muscle toxin which acts on muscular tissue.

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